Anti-TRPML3/Mucolipin-3 [N268/19R] in Western Blot (Rat)

Hybridoma

Data Submitted By: James Trimmer
Lab Name: UC Davis/NIH NeuroMab Facility
Submission Date: March 20, 2024
Publication Date: August 01, 2024 (modified August 01, 2024)
Abstract: Experimental results for Anti-TRPML3/Mucolipin-3 [N268/19R] (Addgene Antibody 209581) in a western blot assay with a rat sample.

Tissue and transfected cell immunoblot: extracts of rat brain membranes (RBM) and of COS cells transiently transfected with
HA-tagged mouse and human TRPML3 (mTRPML3 and hTRPML3) or untagged Kv2.1 plasmid and probed with N268/19 (left) and K89/34 (right) TC supe. — Caption: Tissue and transfected cell immunoblot: extracts of rat brain membranes (RBM) and of COS cells transiently transfected with HA-tagged mouse and human TRPML3 (mTRPML3 and hTRPML3) or untagged Kv2.1 plasmid and probed with N268/19 (left) and K89/34 (right) TC supe.
Image attribution: UC Davis/NIH NeuroMab Facility

Any further use of this image requires permission from the lab who submitted the data.

Related Antibody: Anti-TRPML3/Mucolipin-3 [N268/19R]
Addgene #209581

Materials & Methods

Sample

Species: Rat
Cell / Tissue Type: Brain

Primary Antibody

Name: Anti-TRPML3/Mucolipin-3 [N268/19R]
Species: Mouse
Gene / Target: MCOLN3

Additional Information

Additional Information: NeuroMab hybridoma clone datasheet (Link opens in a new window)
Protocol: Trimmer lab immunoblot labeling protocol (Link opens in a new window)

Results

Result: Pass (The antibody worked under these conditions)