Anti-HA [12CA5] in Western Blot (Human)
Addgene #194541
- Data Submitted By
- Meghan Rego
- Lab Name
- Addgene
- Submission Date
- February 06, 2024
- Publication Date
- February 06, 2024 (modified February 06, 2024)
- Abstract
- Experimental results for Anti-HA [12CA5] (Addgene Antibody 194541) in a western blot assay with a human sample.
![HEK293 cells were left untransfected or transfected (+106410) with pCMV-P-RAN-RFP4. Cell culture supernatant containing the secreted protein was collected and probed with Anti-HA [12CA5] or Anti-6xHis [N144/14R].](https://datahub-media.addgene.org/report-images/u91cpn/194541-Western_Blot-2023-02-02_144242.227634.png)
Image attribution: Addgene
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-HA [12CA5]
Addgene #194541 -
Goat anti-mouse IgG (H+L) HRP
Thermo Fisher #A16078
Materials & Methods
Sample
- Species
- Human
- Cell / Tissue Type
- HEK293
- Total Amount
- 30 µL
Buffer
- Name
- TBS-T
- pH
- 7.4
Blocking
- Agent
- Milk
- Concentration
- 5%
- Time
- 45 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-HA [12CA5]
- Source
- Addgene
- Catalog Number
- 194541
- Species
- Mouse
- Concentration
- 5 µg/mL
- Time
- 45 min
- Temperature
- Room Temperature
Secondary Antibody
- Name
- Goat anti-mouse IgG (H+L) HRP
- Source
- Thermo Fisher
- Catalog Number
- A16078
- Time
- 45 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
- Protocol
- Addgene Western Blot protocol
- Predicted Molecular Weight
- 54 kDa
Results
- Result
- Pass (The antibody worked under these conditions)