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Anti-PV [BCN14.1.1A2] in Immunocytochemistry (Transfected COS)

Addgene #217650

Data Submitted By
James Trimmer
Lab Name
James Trimmer, UC Davis
Submission Date
June 30, 2024
Publication Date
July 30, 2024 (modified December 12, 2024)
DOI
https://doi.org/10.57733/addgene.tzuus1
Abstract
COS cells were transfected with human PV, fixed and labeled with Anti-PV [BCN14.1.1A2] (Addgene #217650) or NeuroMab L114/81. Similar labeling was observed between antibodies.
Citation
Anti-PV [BCN14.1.1A2] in Immunocytochemistry (Transfected COS). Trimmer J 2024. Addgene Report, https://doi.org/10.57733/addgene.tzuus1
Caption: COS cells were transfected with human PV, fixed and labeled with Anti-PV [BCN14.1.1A2] (PV BCN14.1.1A2 R-mAb) or NeuroMab L114/81 (L114/81 mAb). Nuclei were counterstained with Hoechst.
Image attribution: James Trimmer, UC Davis

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibodies Used
Anti-PV [BCN14.1.1A2]
Addgene #217650
Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody
Thermo Fisher #A21131

Materials & Methods

Sample

Species
Human
Cell / Tissue Type
COS
Additional Information
COS cells were transfected with human PV

Buffer

Name
PBS
pH
7.4

Fixative

Fixative
Formaldehyde/Paraformaldehyde
Time
30 min
Temperature
4 ˚C

Blocking

Agent
Milk
Concentration
4% w/v
Time
45 min
Temperature
Room Temperature

Primary Antibody

Name
Anti-PV [BCN14.1.1A2]
Source
Addgene
Catalog Number
217650
Species
Mouse
Concentration
3 µg/mL
Time
45 min
Temperature
Room Temperature
View in Addgene Catalog

Secondary Antibody

Name
Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody
Source
Thermo Fisher
Catalog Number
A21131
Concentration
1:2500
Conjugate
Alexa Fluor 488
Time
45 min
Temperature
Room Temperature

Additional Information

Protocol
Trimmer lab immunocytochemistry protocol
Controls
Labeling of Anti-PV [BCN14.1.1A2] was compared to the previously validated NeuroMab L114/81.

Results

Result
Pass (The antibody worked under these conditions)
Describe Results
Equivalent signal strength and labeling pattern between antibodies