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Anti-PV [BCN14.1.1A2] in Immunocytochemistry (Transfected COS)

Addgene #217650

Data Submitted By: James Trimmer
Lab Name: James Trimmer, UC Davis
Submission Date: June 30, 2024
Publication Date: July 30, 2024 (modified August 21, 2024)
Abstract: COS cells were transfected with human PV, fixed and labeled with Anti-PV [BCN14.1.1A2] (Addgene #217650) or NeuroMab L114/81. Similar labeling was observed between antibodies.

COS cells were transfected with human PV, fixed and labeled with Anti-PV [BCN14.1.1A2] (PV BCN14.1.1A2 R-mAb) or NeuroMab L114/81 (L114/81 mAb). Nuclei were counterstained with Hoechst. — Caption: COS cells were transfected with human PV, fixed and labeled with Anti-PV [BCN14.1.1A2] (PV BCN14.1.1A2 R-mAb) or NeuroMab L114/81 (L114/81 mAb). Nuclei were counterstained with Hoechst.
Image attribution: James Trimmer, UC Davis

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibodies Used: Anti-PV [BCN14.1.1A2]
Addgene #217650; Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody
Thermo Fisher #A21131

Materials & Methods

Sample

Species: Primate
Cell / Tissue Type: COS
Additional Information: COS cells were transfected with human PV

Buffer

Name: PBS
pH: 7.4

Fixative

Fixative: Formaldehyde/Paraformaldehyde
Time: 30 min
Temperature: 4 ˚C

Blocking

Agent: Milk
Concentration: 4% w/v
Time: 45 min
Temperature: Room Temperature

Primary Antibody

Name: Anti-PV [BCN14.1.1A2]
Source: Addgene
Catalog Number: 217650
Species: Mouse
Concentration: 3 µg/mL
Time: 45 min
Temperature: Room Temperature

View in Addgene Catalog

Secondary Antibody

Name: Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody
Source: Thermo Fisher
Catalog Number: A21131
Concentration: 1:2500
Conjugate: Alexa Fluor 488
Time: 45 min
Temperature: Room Temperature

Additional Information

Protocol: Trimmer lab immunocytochemistry protocol
Controls: Labeling of Anti-PV [BCN14.1.1A2] was compared to the previously validated NeuroMab L114/81.

Results

Result: Pass (The antibody worked under these conditions)
Describe Results: Equivalent signal strength and labeling pattern between antibodies