Anti-Pan-Nav channel [K58/35] - Chimeric in Western Blot (Rat)
Addgene #196563
- Data Submitted By
- James Trimmer
- Lab Name
- James Trimmer, UC Davis
- Submission Date
- February 06, 2024
- Publication Date
- February 06, 2024 (modified February 06, 2024)
- Abstract
- Experimental results for Anti-Pan-Nav channel [K58/35] - Chimeric (Addgene Antibody 196563) in a western blot assay with a rat sample.
![Crude rat membranes immunoblotted against Anti-Pan-Nav channel [K58/35] mouse hybridoma from the Trimmer lab (H), mouse recombinant antibody (R, Addgene: 191809) or rabbit chimeric antibody (R*, Addgene: 196563).](https://datahub-media.addgene.org/report-images/txxjvv/196563-Western_Blot-2023-10-24_152504.239817.png)
Image attribution: James Trimmer, UC Davis
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibody Used
-
Anti-Pan-Nav channel [K58/35] - Chimeric
Addgene #196563
Materials & Methods
Sample
- Species
- Rat
- Cell / Tissue Type
- Brain
Buffer
- Name
- TBS
- pH
- 7.5
Blocking
- Agent
- Milk
- Concentration
- 4%
- Time
- 45 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-Pan-Nav channel [K58/35] - Chimeric
- Source
- Addgene
- Catalog Number
- 196563
- Species
- Rabbit
- Gene / Target
- SCN1A
- Concentration
- 1 µg/mL
- Time
- 45 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
Total rat brain extract was incubated at 37 °C for 15 min and separated by denaturing SDS-PAGE. Samples were not boiled prior to loading.
Secondary antibody: Goat anti-rabbit HRP secondary, diluted to 1:10,000 or goat anti-mouse IgG2a HRP secondary, diluted to 1:25,000 in 4% non-fat dry milk in TBS pH 7.5, 45 min, room temperature
- Protocol
- Addgene Western Blot protocol
- Predicted Molecular Weight
- >200 kDa
Results
- Result
- Pass (The antibody worked under these conditions)