Anti-MMACHC [N230/21R] in Western Blot

Hybridoma

Data Submitted By: James Trimmer
Lab Name: UC Davis/NIH NeuroMab Facility
Submission Date: March 20, 2024
Publication Date: August 01, 2024 (modified August 01, 2024)
Abstract: Experimental results for Anti-MMACHC [N230/21R] (Addgene Antibody 209579) in a western blot assay with a green monkey sample.

Transfected cell immunoblot: extracts of COS cells transiently
transfected with Myc-tagged MMACHC or untagged Kv2.1
plasmid and probed with N230/21 (left) and N52A/42 (right) TC
supe. — Caption: Transfected cell immunoblot: extracts of COS cells transiently transfected with Myc-tagged MMACHC or untagged Kv2.1 plasmid and probed with N230/21 (left) and N52A/42 (right) TC supe.
Image attribution: UC Davis/NIH NeuroMab Facility

Any further use of this image requires permission from the lab who submitted the data.

Related Antibody: Anti-MMACHC [N230/21R]
Addgene #209579

Materials & Methods

Sample

Species: Green monkey
Cell / Tissue Type: COS

Primary Antibody

Name: Anti-MMACHC [N230/21R]
Species: Mouse
Gene / Target: MMACHC

Additional Information

Additional Information: NeuroMab hybridoma clone datasheet (Link opens in a new window)
Protocol: Trimmer lab immunoblot labeling protocol (Link opens in a new window)

Results

Result: Pass (The antibody worked under these conditions)