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Anti-GST [N100/13R ] in Western Blot

Addgene #192032

Data Submitted By: Meghan Rego
Lab Name: Addgene
Submission Date: February 06, 2024
Publication Date: February 06, 2024 (modified February 06, 2024)
Abstract: Experimental results for Anti-GST [N100/13R ] (Addgene Antibody 192032) in a western blot assay with a other sample.

E. coli were left untreated or transformed with plasmid # 178975 GST-His8-GFP-nanobody (+ 178975). After induction, E. coli were harvested, lysed, and separated by denaturing SDS-PAGE and immunoblotted with Anti-GST [N100/13R ] or Anti-6xHis [N144/14R]. — Caption: E. coli were left untreated or transformed with plasmid # 178975 GST-His8-GFP-nanobody (+ 178975). After induction, E. coli were harvested, lysed, and separated by denaturing SDS-PAGE and immunoblotted with Anti-GST [N100/13R ] or Anti-6xHis [N144/14R].
Image attribution: Addgene

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibodies Used: Anti-GST [N100/13R ]
Addgene #192032; goat anti-mouse IgG (H+L) HRP
Thermo Fisher #A16078

Materials & Methods

Sample

Species: Other
Cell / Tissue Type: E. coli
Total Amount: 30 µg

Buffer

Name: TBS-T
pH: 7.4

Blocking

Agent: BSA
Concentration: 5%
Time: 45 min
Temperature: Room Temperature

Primary Antibody

Name: Anti-GST [N100/13R ]
Source: Addgene
Catalog Number: 192032
Species: Mouse
Concentration: 10 µg/mL
Time: overnight
Temperature: 4 °C

View in Addgene Catalog

Secondary Antibody

Name: goat anti-mouse IgG (H+L) HRP
Source: Thermo Fisher
Catalog Number: A16078
Time: 45 min
Temperature: Room Temperature

Additional Information

Additional Information
Protocol: Addgene Western Blot protocol
Predicted Molecular Weight: 41 kDa

Results

Result: Pass (The antibody worked under these conditions)