Anti-GABA(A)R, Alpha5 [N415/24R] in Western Blot (Rat)

Hybridoma

Data Submitted By: James Trimmer
Lab Name: UC Davis/NIH NeuroMab Facility
Submission Date: February 06, 2024
Publication Date: February 06, 2024 (modified March 18, 2024)
Abstract: Experimental results for Anti-GABA(A)R, Alpha5 [N415/24R] (hybridoma-derived) in a western blot assay with a rat sample.

Immunoblot against crude low-speed pellet fractions from adult rat brain (RBLSP) and wild-type (WT) and GABA-A-RAlpha5 knockout (KO) mouse brains probed with N415/24 (left) and N52A/42 (right) TC supe. — Caption: Immunoblot against crude low-speed pellet fractions from adult rat brain (RBLSP) and wild-type (WT) and GABA-A-RAlpha5 knockout (KO) mouse brains probed with N415/24 (left) and N52A/42 (right) TC supe.
Image attribution: UC Davis/NIH NeuroMab Facility

Any further use of this image requires permission from the lab who submitted the data.

Related Antibody: Anti-GABA(A)R, Alpha5 [N415/24R]
Addgene #184218

Materials & Methods

Sample

Species: Rat
Cell / Tissue Type: Brain

Primary Antibody

Name: Anti-GABA(A)R, Alpha5 [N415/24R]
Species: Mouse
Gene / Target: GABRA5

Additional Information

Additional Information: NeuroMab hybridoma clone datasheet (Link opens in a new window)

Results

Result: Pass (The antibody worked under these conditions)
Knockout Validation: Yes