Anti-Iduna/RNF146 [N201/35R] in Western Blot (Rat)
Addgene #180112
- Data Submitted By
- Meghan Rego
- Lab Name
- Addgene
- Submission Date
- February 06, 2024
- Publication Date
- February 06, 2024 (modified February 06, 2024)
- Abstract
- Experimental results for Anti-Iduna/RNF146 [N201/35R] (Addgene Antibody 180112) in a western blot assay with a rat sample.
Image attribution: Addgene
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-Iduna/RNF146 [N201/35R]
Addgene #180112 -
goat anti-mouse IgG (H+L) HRP
Thermo Fisher #A16078
Materials & Methods
Sample
- Species
- Rat
- Cell / Tissue Type
- Brain
Buffer
- Name
- TBS
- pH
- 7.5
Blocking
- Agent
- Milk
- Concentration
- 4%
- Time
- 45 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-Iduna/RNF146 [N201/35R]
- Source
- Addgene
- Catalog Number
- 180112
- Species
- Mouse
- Gene / Target
- Rnf146
- Concentration
- 0.67 µg/mL
- Time
- 45 min
- Temperature
- Room Temperature
Secondary Antibody
- Name
- goat anti-mouse IgG (H+L) HRP
- Source
- Thermo Fisher
- Catalog Number
- A16078
- Time
- 45 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
Total rat brain extract was reduced and separated by denaturing SDS-PAGE
- Protocol
- Addgene Western Blot protocol
- Predicted Molecular Weight
- 40 kDa
Results
- Result
- Pass (The antibody worked under these conditions)