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Anti-Iduna/RNF146 [N201/35R] in Western Blot (Rat)

Addgene #180112

Data Submitted By
Meghan Rego
Lab Name
Addgene
Submission Date
February 06, 2024
Publication Date
February 06, 2024 (modified February 06, 2024)
Abstract
Experimental results for Anti-Iduna/RNF146 [N201/35R] (Addgene Antibody 180112) in a western blot assay with a rat sample.
Caption: Rat brain lysates were immunoblotted against purified recombinant antibody produced at Addgene.
Image attribution: Addgene

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibodies Used
Anti-Iduna/RNF146 [N201/35R]
Addgene #180112
goat anti-mouse IgG (H+L) HRP
Thermo Fisher #A16078

Materials & Methods

Sample

Species
Rat
Cell / Tissue Type
Brain

Buffer

Name
TBS
pH
7.5

Blocking

Agent
Milk
Concentration
4%
Time
45 min
Temperature
Room Temperature

Primary Antibody

Name
Anti-Iduna/RNF146 [N201/35R]
Source
Addgene
Catalog Number
180112
Species
Mouse
Gene / Target
Rnf146
Concentration
0.67 µg/mL
Time
45 min
Temperature
Room Temperature
View in Addgene Catalog

Secondary Antibody

Name
goat anti-mouse IgG (H+L) HRP
Source
Thermo Fisher
Catalog Number
A16078
Time
45 min
Temperature
Room Temperature

Additional Information

Additional Information

Total rat brain extract was reduced and separated by denaturing SDS-PAGE

Protocol
Addgene Western Blot protocol
Predicted Molecular Weight
40 kDa

Results

Result
Pass (The antibody worked under these conditions)