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Anti-CASK [K56A/50R] in Western Blot (Rat)

Addgene #183002

Data Submitted By: Meghan Rego
Lab Name: Addgene
Submission Date: February 06, 2024
Publication Date: February 06, 2024 (modified February 06, 2024)
Abstract: Experimental results for Anti-CASK [K56A/50R] (Addgene Antibody 183002) in a western blot assay with a rat sample.

Crude rat membranes immunoblotted against Anti-CASK purified recombinant antibody produced at Addgene (R) or hybridoma-derived antibody from the Trimmer Lab at UC Davis (H). Blots were stripped and reprobed with anti-vinculin as a loading control. — Caption: Crude rat membranes immunoblotted against Anti-CASK purified recombinant antibody produced at Addgene (R) or hybridoma-derived antibody from the Trimmer Lab at UC Davis (H). Blots were stripped and reprobed with anti-vinculin as a loading control.
Image attribution: Addgene

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibodies Used: Anti-CASK [K56A/50R]
Addgene #183002; goat anti-mouse IgG (H+L) HRP
Thermo Fisher #A16078

Materials & Methods

Sample

Species: Rat
Cell / Tissue Type: Brain
Total Amount: 30 µg

Buffer

Name: TBS-T
pH: 7.4

Blocking

Agent: Milk
Concentration: 5%
Time: 45 min
Temperature: Room Temperature

Primary Antibody

Name: Anti-CASK [K56A/50R]
Source: Addgene
Catalog Number: 183002
Species: Mouse
Gene / Target: CASK
Concentration: 1 µg/mL
Time: 45 min
Temperature: Room Temperature

View in Addgene Catalog

Secondary Antibody

Name: goat anti-mouse IgG (H+L) HRP
Source: Thermo Fisher
Catalog Number: A16078
Time: 45 min
Temperature: Room Temperature

Additional Information

Additional Information
Protocol: Addgene Western Blot protocol
Predicted Molecular Weight: 103 kDa

Results

Result: Pass (The antibody worked under these conditions)