Anti-Jagged [D5] in Immunocytochemistry (Human)
Addgene #201864
- Data Submitted By
- Amrita Rhoads
- Lab Name
- Addgene
- Submission Date
- February 06, 2024
- Publication Date
- February 06, 2024 (modified March 29, 2024)
- Abstract
- Experimental results for Anti-Jagged [D5] (Addgene Antibody 201864) in an immunocytochemistry assay with a human sample.
![HeLa cells were untransfected or transiently transfected with Jag1-HA pIRES (+17336) and stained with Anti-Jagged [D5] 96 h later.](https://datahub-media.addgene.org/report-images/p1acwx/201864-Immunocytochemistry-2023-10-27_104613.135145.png)
Image attribution: Addgene
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-Jagged [D5]
Addgene #201864 -
Goat anti-human IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 594
Thermo Fisher #A11014
Materials & Methods
Sample
- Species
- Human
- Cell / Tissue Type
- HeLa
Buffer
- Name
- PBS
Fixative
- Fixative
- Formaldehyde/Paraformaldehyde
Permeabilization
- Agent
- 0.2% Triton X-100 in PBS
- Time
- 10 min
- Temperature
- Room Temperature
Blocking
- Agent
- BSA
- Concentration
- 5%
- Time
- 20 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-Jagged [D5]
- Source
- Addgene
- Catalog Number
- 201864
- Species
- Human
- Gene / Target
- JAG1
- Concentration
- 0.1 µg/mL
- Time
- 60 min
- Temperature
- Room Temperature
Secondary Antibody
- Name
- Goat anti-human IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 594
- Source
- Thermo Fisher
- Catalog Number
- A11014
- Concentration
- 2 µg/mL
- Time
- 30 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
HeLa cells were untransfected or transiently transfected with Jag1-HA pIRES and stained 96 h later with Anti-Jagged [D5]
- Protocol
- Addgene immunocytochemistry protocol
Results
- Result
- Pass (The antibody worked under these conditions)