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Anti-Kvbeta1.2/KCNAB1 K+ channel [K47/42R] in Western Blot (Rat)

Addgene #209588

Data Submitted By
James Trimmer
Lab Name
James Trimmer, UC Davis
Submission Date
September 10, 2024
Publication Date
October 11, 2024 (modified October 18, 2024)
Abstract
Experimental results for Kvbeta1.2/KCNAB1 K+ channel [K47/42R] (Addgene #209588) in a western blot assay with a rat brain lysate. The labeling by the Anti-Kvbeta1.2/KCNAB1 K+ channel recombinant antibody produced at Addgene (R) was compared to a UC Davis produced lot (R').
Caption: Crude rat membranes immunoblotted against Anti-Kvbeta1.2/KCNAB1 K+ channel [K47/42R] from the Trimmer Lab at UC Davis (R') or antibody produced at Addgene (R).
Image attribution: James Trimmer, UC Davis

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibodies Used
Anti-Kvbeta1.2/KCNAB1 K+ channel [K47/42R]
Addgene #209588
Goat anti-mouse IgG2a
Jackson ImmunoResearch Labs #115-035-206

Materials & Methods

Sample

Species
Rat
Cell / Tissue Type
Brain
Additional Information
Rat brain lysates were boiled at 95 °C 5 min prior to loading.

Buffer

Name
TBS
pH
7.5

Blocking

Agent
Milk
Concentration
4% w/v
Time
45 min
Temperature
Room Temperature

Primary Antibody

Name
Anti-Kvbeta1.2/KCNAB1 K+ channel [K47/42R]
Source
Addgene
Catalog Number
209588
Species
Mouse
Gene / Target
KCNAB1
Concentration
1 µg/mL
Time
45 min
Temperature
Room Temperature
View in Addgene Catalog

Secondary Antibody

Name
Goat anti-mouse IgG2a
Source
Jackson ImmunoResearch Labs
Catalog Number
115-035-206
Concentration
1:8,333
Conjugate
HRP
Time
45 min
Temperature
Room Temperature

Additional Information

Additional Information
Total rat brain extract was reduced and separated by denaturing SDS-PAGE.
Protocol
Trimmer lab immunoblot labeling protocol (Link opens in a new window)
Controls
Compared labeling by Addgene-produced recombinant antibody (#209588) in western blot (Rat) to the validated UC Davis-produced recombinant antibody.
Detection Method
ECL
Predicted Molecular Weight
65 kDa

Results

Result
Pass (The antibody worked under these conditions)
Describe Results
Equivalent signal strength and labeling pattern between the UC Davis and Addgene-produced antibodies.