Anti-PV [BCN14.1.1A2] in Immunohistochemistry (Rat)
Addgene #217650
- Data Submitted By
- James Trimmer
- Lab Name
- James Trimmer, UC Davis
- Submission Date
- June 30, 2024
- Publication Date
- July 30, 2024 (modified August 21, 2024)
- Abstract
- Experimental results for Anti-PV [BCN14.1.1A2] (Addgene #217650) in an immunohistochemistry assay with a rat cortex sample.
![Free-floating rat brain cortex sections were fixed and labeled with purified Anti-PV [BCN14.1.1A2] or NeuroMab clone L114/3.3. The labeling patterns were compared between the two antibodies.](https://datahub-media.addgene.org/report-images/oq9yjc/217650cortex.png)
Image attribution: James Trimmer, UC Davis
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-PV [BCN14.1.1A2]
Addgene #217650 -
Mix of goat biotinylated anti-mouse IgG antibodies
Jackson ImmunoResearch Labs #115-065-205, 115-065-206, 115-065-207
Materials & Methods
Sample
- Species
- Rat
- Cell / Tissue Type
- Brain
- Preparation
- Free-floating
Buffer
- Name
- PB
- pH
- 7.4
Fixative
- Fixative
- Formaldehyde/Paraformaldehyde
Permeabilization / Delipidation
- Agent
- PBS containing 1:2000 avidin and 0.5% Triton X-100
- Time
- 45 min
- Temperature
- Room Temperature
Blocking
- Agent
- Serum
- Concentration
- 0.50%
- Time
- 45 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-PV [BCN14.1.1A2]
- Source
- Addgene
- Catalog Number
- 217650
- Species
- Mouse
- Concentration
- 1 µg/mL
- Time
- Overnight
- Temperature
- 4 ˚C
Secondary Antibody
- Name
- Mix of goat biotinylated anti-mouse IgG antibodies
- Source
- Jackson ImmunoResearch Labs
- Catalog Number
- 115-065-205, 115-065-206, 115-065-207
- Concentration
- 0.5 µg/mL
- Time
- 60 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
- Goat serum used as blocking agent.
- Protocol
- Trimmer lab immunohistochemistry protocol
- Controls
- The labeling pattern was compared to the NeuroMab L114/3.3
Results
- Result
- Pass (The antibody worked under these conditions)
- Describe Results
- Labeling pattern was compared and found to be equivalent to the NeuroMab L114/3.3