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Anti-PV [BCN14.1.1A2] in Immunohistochemistry (Rat)

Addgene #217650

Data Submitted By
James Trimmer
Lab Name
James Trimmer, UC Davis
Submission Date
June 30, 2024
Publication Date
July 30, 2024 (modified August 21, 2024)
Abstract
Experimental results for Anti-PV [BCN14.1.1A2] (Addgene #217650) in an immunohistochemistry assay with a rat sample.
Caption: Free-floating rat brain sections were fixed and labeled with purified Anti-PV [BCN14.1.1A2] or NeuroMab clone L114/3.3. The labeling patterns were compared between the two antibodies.
Image attribution: James Trimmer, UC Davis

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibodies Used
Anti-PV [BCN14.1.1A2]
Addgene #217650
Mix of goat biotinylated anti-mouse IgG antibodies
Jackson ImmunoResearch Labs #115-065-205, 115-065-206, 115-065-207

Materials & Methods

Sample

Species
Rat
Cell / Tissue Type
Brain
Preparation
Free-floating

Buffer

Name
PB
pH
7.4

Fixative

Fixative
Formaldehyde/Paraformaldehyde

Permeabilization / Delipidation

Agent
PBS containing 1:2000 avidin and 0.5% Triton X-100
Time
45 min
Temperature
Room Temperature

Blocking

Agent
Serum
Concentration
0.50%
Time
45 min
Temperature
Room Temperature

Primary Antibody

Name
Anti-PV [BCN14.1.1A2]
Source
Addgene
Catalog Number
217650
Species
Mouse
Concentration
1 µg/mL
Time
Overnight
Temperature
Room Temperature
View in Addgene Catalog

Secondary Antibody

Name
Mix of goat biotinylated anti-mouse IgG antibodies
Source
Jackson ImmunoResearch Labs
Catalog Number
115-065-205, 115-065-206, 115-065-207
Concentration
0.5 µg/mL
Time
60 min
Temperature
Room Temperature

Additional Information

Additional Information
Goat serum used as blocking agent.
Protocol
Trimmer lab immunohistochemistry protocol
Controls
The labeling pattern was compared to the NeuroMab L114/3.3

Results

Result
Pass (The antibody worked under these conditions)
Describe Results
Labeling pattern was compared and found to be equivalent to the NeuroMab L114/3.3