ICC for Anti-DCC [IPI-mDCC.114] in cortical neurons
Addgene #241890
- Data Submitted By
- Teddy AJ Fisher and Timothy Kennedy
- Lab Name
- Timothy Kennedy, McGill University
- Submission Date
- March 06, 2026
- Publication Date
- March 23, 2026 (modified March 23, 2026)
- DOI
- https://doi.org/10.57733/addgene.nk4yn3
- Abstract
- Experimental results for Anti-DCC [IPI-mDCC.114] (Addgene #241890) in an immunocytochemistry (ICC) assay with rat cortical neurons.
- Citation
-
ICC for Anti-DCC [IPI-mDCC.114] in cortical neurons. Fisher T, Kennedy T 2026. Addgene Report, https://doi.org/10.57733/addgene.nk4yn3
![Immunohistochemistry (ICC) analysis of 14 DIV rat E18 cortical neurons. Labeling for DCC was performed using Anti-DCC [IPI-mDCC.114] (Addgene #241890). Positive control (+) was performed using a validated commercial antibody (Abcam, #ab27350). Negative control (-) was performed using only the secondary antibody.](https://datahub-media.addgene.org/report-images/nk4yn3/DCC.114-Kennedy-ICC.png)
Image attribution: Timothy Kennedy, McGill University
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-DCC [IPI-mDCC.114]
Addgene #241890 -
Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 555
Thermo Fisher #A31572
Materials & Methods
Sample
- Target Species
- Rat
- Cell / Tissue Type
- E18 cortical neurons
- Additional Information
- E18 cells at DIV 14 in culture
Buffer
- Name
- PBS
Fixative
- Fixative
- 4% PFA in PBS
- Time
- 15 min
- Temperature
- Room Temperature
Permeabilization
- Agent
- Triton X-100
- Concentration
- 0.25%
- Time
- 15 min
- Temperature
- Room Temperature
Blocking
- Agent
- PBS, 5% BSA, 5% Heat inactivated horse serum, 0.25% Triton X-100
- Time
- 15 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-DCC [IPI-mDCC.114]
- Source
- Addgene
- Catalog Number
- 241890
- Host Species
- Rabbit
- Target Antigen
- DCC
- Concentration
- 2 µg/mL
- Time
- Overnight
- Temperature
- 4 ˚C
Secondary Antibody
- Name
- Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 555
- Source
- Thermo Fisher
- Catalog Number
- A31572
- Concentration
- 0.736 µg/mL
- Time
- 60 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
Imaged on a Zeiss 880 confocal microscope.
Positive control was an identical sample processed identically in parallel with the commercially available Anti-DCC antibody [EPR23313-115] (Abcam, #ab273570), which is also a rabbit monoclonal antibody. This antibody has been KO validated by the Kennedy lab. The same secondary antibody was used for the positive control.
Negative control was performed using only the secondary antibody.
Results
- Result
- Pass (The antibody worked under these conditions)
- Describe Results
- Positive labeling for DCC, consistent with results from a previously validated antibody.