Anti-HCN1 [N70/28R] in Western Blot (Mouse)

Hybridoma

Data Submitted By: James Trimmer
Lab Name: UC Davis/NIH NeuroMab Facility
Submission Date: February 06, 2024
Publication Date: February 06, 2024 (modified March 18, 2024)
Abstract: Experimental results for Anti-HCN1 [N70/28R] (hybridoma-derived) in a western blot assay with a mouse sample.

Adult rat and mouse brain membrane (RBM and MBM, respectively) and transfected cell immunoblot: extracts of RBM, HCN1-KO and -WT MBM and COS-1 cells transfected with GFP-HCN1, GFP-HCN2 or Kv2.1 plasmids and probed with N70/28 (left panel) or K89/34 TC supe (right panel). — Caption: Adult rat and mouse brain membrane (RBM and MBM, respectively) and transfected cell immunoblot: extracts of RBM, HCN1-KO and -WT MBM and COS-1 cells transfected with GFP-HCN1, GFP-HCN2 or Kv2.1 plasmids and probed with N70/28 (left panel) or K89/34 TC supe (right panel).

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Related Antibody: Anti-HCN1 [N70/28R]
Addgene #180092

Materials & Methods

Sample

Species: Mouse
Cell / Tissue Type: Brain membrane

Primary Antibody

Name: Anti-HCN1 [N70/28R]
Species: Mouse
Gene / Target: Hcn1

Additional Information

Additional Information: NeuroMab hybridoma clone datasheet (Link opens in a new window)

Results

Result: Pass (The antibody worked under these conditions)
Knockout Validation: Yes