Anti-Kir6.2 K+ channel [N363/71R] in Western Blot (Transfected COS)

Hybridoma

Data Submitted By: James Trimmer
Lab Name: UC Davis/NIH NeuroMab Facility
Submission Date: March 20, 2024
Publication Date: October 21, 2024 (modified October 21, 2024)
Abstract: Experimental results for Anti-Kir6.2 K+ channel [N363/71R] (hybridoma-derived) in a western blot assay with COS cells transfected with HA-Kir6.2 K+ channel.

Immunoblot against extracts of COS cells transiently transfected with Myc-tagged Kir6.1, HA-tagged Kir6.2 or untagged Kv2.1 plasmid probed with N363/71 (left) or N52A/42 (right) TC supe. — Caption: Immunoblot against extracts of COS cells transiently transfected with Myc-tagged Kir6.1, HA-tagged Kir6.2 or untagged Kv2.1 plasmid probed with N363/71 (left) or N52A/42 (right) TC supe.
Image attribution: UC Davis/NIH NeuroMab Facility

Any further use of this image requires permission from the lab who submitted the data.

Related Antibody: Anti-Kir6.2 K+ channel [N363/71R]
Addgene #209595

Materials & Methods

Sample

Species: Green monkey
Cell / Tissue Type: COS

Primary Antibody

Name: Anti-Kir6.2 K+ channel [N363/71R]
Species: Mouse
Gene / Target: Kcnj11

Additional Information

Additional Information: NeuroMab hybridoma clone datasheet (Link opens in a new window)
Protocol: Trimmer lab immunoblot labeling protocol (Link opens in a new window)

Results

Result: Pass (The antibody worked under these conditions)