Skip to main content

Anti-Integrin alpha M [IPI-LM2/1] in Flow Cytometry (Human)

Addgene #213678

Data Submitted By
IPI Staff
Lab Name
Institute for Protein Innovation (IPI)
Submission Date
March 25, 2024
Publication Date
March 25, 2024 (modified March 27, 2024)
Abstract
Experimental results for Anti-Integrin alpha M [IPI-LM2/1] (Addgene Antibody 213678) in a flow cytometry assay using Expi CHO cells transiently transfected with human integrin αMβ2.
Caption: Bar graph of the relative mean fluorescence intensity (MFI) response of the LM2/1 antibody to Expi CHO cells transfected with B7H3 (Control) or human integrin αMβ2 (Human αM/β2). Cells were labeled with the hybridoma monoclonal antibody LM2/1 (Hybridoma) from Biolegend (Catalog number 393102), recombinant mouse IgG2a LM2/1 antibody (Mouse IgG2a, #213678, orange), or mouse/rabbit IPI-LM2/1 antibody (Rabbit IgG1, #213668) and species-specific Alexa Fluor 647-conjugated secondary antibodies.
Image attribution: Institute for Protein Innovation (IPI)

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibody Used
Anti-Integrin alpha M [IPI-LM2/1]
Addgene #213678

Materials & Methods

Sample

Species
Human
Cell / Tissue Type
Expi CHO cells
Additional Information
Expi CHO cells were transiently transfected with B7H3 (Control) or human integrin αMβ2.

Primary Antibody

Name
Anti-Integrin alpha M [IPI-LM2/1]
Source
Addgene
Catalog Number
213678
Species
Mouse
Gene / Target
ITGAM
Concentration
10 µg/mL
Time
30 min
Temperature
4 ˚C
View in Addgene Catalog

Additional Information

Additional Information

This data compares labeling by the rabbit chimera recombinant version of the IPI-LM2/1 antibody (Addgene #213668) and the mouse recombinant version (Addgene #213678) to a hybridoma version (Biolegend, Catalog number 393102). Use of the hybridoma antibody was first reported in (Miller et al. 1986).

Results

Result
Pass (The antibody worked under these conditions)