Anti-CD4 [mu5A8] in Western Blot (Human)
Addgene #212327
- Data Submitted By
- Amrita Rhoads
- Lab Name
- Addgene
- Submission Date
- April 26, 2024
- Publication Date
- May 31, 2024 (modified June 07, 2024)
- Abstract
- Experimental results for Anti-CD4 [mu5A8] (Addgene #212327) in a western blot assay with a human sample.
Image attribution: Amrita Rhoads
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-CD4 [mu5A8]
Addgene #212327 -
Goat anti-mouse IgG (H+L) HRP
Thermo Fisher #A16078
Materials & Methods
Sample
- Species
- Human
- Cell / Tissue Type
- HEK293
- Additional Information
- HEK293 cell lysates expressing CD4-DDK (Origene, catalog number LC400209) or CD41-DDK (Origene, catalog number LC424734) were obtained from Origene.
- Total Amount
- 10 µg
Buffer
- Name
- TBS-T
- pH
- 7.4
Blocking
- Agent
- Milk
- Concentration
- 5%
- Time
- 60 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-CD4 [mu5A8]
- Source
- Addgene
- Catalog Number
- 212327
- Species
- Mouse
- Gene / Target
- CD4
- Concentration
- 4 µg/mL
- Time
- Overnight
- Temperature
- 4 ˚C
Secondary Antibody
- Name
- Goat anti-mouse IgG (H+L) HRP
- Source
- Thermo Fisher
- Catalog Number
- A16078
- Species
- Goat
- Concentration
- 0.4 µg/mL
- Conjugate
- HRP
- Time
- 45 min
- Temperature
- Room Temperature
Additional Information
- Protocol
- Addgene Western Blot protocol
- Controls
- Anti-Vinculin FB11 (Enzo, catalog number BMLVG61100100) was used as a loading control and anti-DDK (Addgene #194502) was used as a positive control.
- Detection Method
- ECL
- Predicted Molecular Weight
- 51.1 kDa
Results
- Result
- Pass (The antibody worked under these conditions)