Anti-Neuroligin-2 [IPI-NLGN2.13] in Immunohistochemistry (rat)
Addgene #219621
- Data Submitted By
- Karl Murray
- Lab Name
- NIH NeuroMab, UC Davis
- Submission Date
- March 03, 2026
- Publication Date
- March 23, 2026 (modified March 23, 2026)
- DOI
- https://doi.org/10.57733/addgene.l4r044
- Abstract
- Experimental results for Anti-Neuroligin-2 [IPI-NLGN2.13] (Addgene#219621) in an immunohistochemistry assay with a rat sample.
- Citation
-
Anti-Neuroligin-2 [IPI-NLGN2.13] in Immunohistochemistry (rat). Murray K 2026. Addgene Report, https://doi.org/10.57733/addgene.l4r044
![Free-floating rat brain sections were fixed and labeled with Anti-Neuroligin-2 [IPI-NLGN2.13]. Hippocampal labeling is shown. Abbreviations: DG: dentate gyrus; CA3: cornu ammonis 3; CA2: cornu ammonis 2; CA1: cornu ammonis 1; sg: dentate gyrus stratum granulosum; ml: molecular layer; sp: stratum pyramidale; sr: stratum radiatum; so: stratum oriens.](https://datahub-media.addgene.org/report-images/l4r044/NLGN2.13_NeuroMab_IHC.png)
Image attribution: NIH NeuroMab
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-Neuroligin-2 [IPI-NLGN2.13]
Addgene #219621 -
Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated
Vector Laboratories #BA-1000-1
Materials & Methods
Sample
- Target Species
- Rat
- Cell / Tissue Type
- Brain
- Additional Information
- 30 µm section
- Preparation
- Free-floating
Buffer
- Name
- TBS (0.15 M NaCl, 50 mM Tris)
- pH
- 7.5
Fixative
- Fixative
- Paraformaldehyde, 4%
Permeabilization / Delipidation
- Agent
- TBS with 0.5% Triton X-100, 0.01 mg/mL Avidin
- Time
- 45 min
- Temperature
- 4 ˚C
Blocking
- Agent
- TBS with 5% normal goat serum, 0.1% Triton X-100, 0.01875 mg/mL Biotin
- Time
- 45 min
- Temperature
- 4 ˚C
Primary Antibody
- Name
- Anti-Neuroligin-2 [IPI-NLGN2.13]
- Source
- Addgene
- Catalog Number
- 219621
- Host Species
- Rabbit
- Target Antigen
- NLGN2
- Concentration
- 5 µg/mL (1:200 dilution)
- Time
- Overnight
- Temperature
- 4 ˚C
Secondary Antibody
- Name
- Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated
- Source
- Vector Laboratories
- Catalog Number
- BA-1000-1
- Concentration
- 0.5 µg/mL
- Time
- 60 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
Validated by comparing to expression levels from in situ hybridization done by Allen Brain Atlas (Link opens in a new window) from a sagittal section of P56 mouse brain.
- Protocol
- Trimmer lab immunohistochemistry protocol (Link opens in a new window)
Results
- Result
- Pass (The antibody worked under these conditions)
- Describe Results
- Strong signal for NLGN2