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Anti-Integrin alpha 4 [IPI-9C10] in Flow Cytometry (Mouse)

Addgene #213670

Data Submitted By
IPI Staff
Lab Name
Institute for Protein Innovation (IPI)
Submission Date
March 20, 2024
Publication Date
June 12, 2024 (modified June 12, 2024)
Abstract
Experimental results for Anti-Integrin alpha 4 [IPI-9C10] (Addgene #213670) in a flow cytometry assay with a mouse sample.
Caption: Flow cytometry data showing positive labeling by Anti-Integrin alpha 4 [IPI-9C10] antibody (Addgene #213670) in mouse CD4+ T cells, B cells, and NK cells. Mouse splenocytes were obtained from a commercial source and labeled with Anti-Integrin alpha 4 [IPI-9C10] conjugated with BroadPharma Fluor 488 NHS Ester (BP488). Control samples were labeled with BP488-conjugated rabbit isotype control (Cell Signaling Technologies #3900S). The cells were also labeled with cell type markers to distinguish leukocytes (CD45), T cells (CD3), cytotoxic T cells (CD3/CD8), helper T cells (CD3/CD4), B cells (CD19), and NK cells (CD49). After labeling and washing, flow cytometry experiments were performed on a CytoFLEX flow cytometer.
Image attribution: Institute for Protein Innovation (IPI)

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibody Used
Anti-Integrin alpha 4 [IPI-9C10]
Addgene #213670

Materials & Methods

Sample

Species
Mouse
Cell / Tissue Type
Splenocytes

Blocking

Agent
1% BSA with Fc blocker
Time
20 min
Temperature
4 ˚C

Primary Antibody

Name
Anti-Integrin alpha 4 [IPI-9C10]
Source
Addgene
Catalog Number
213670
Species
Rabbit
Gene / Target
Itga4
Concentration
10 µg/mL
Conjugate
BroadPharma Fluor 488 NHS Ester (BP488)
Time
30 min
Temperature
4 ˚C
View in Addgene Catalog

Additional Information

Additional Information

Mouse Splenocytes were obtained from a commercial source.

Detection: Flow cytometry experiments were performed on a CytoFLEX flow cytometer. The cells were also labeled with cell type markers to distinguish leukocytes (CD45), T cells (CD3), cytotoxic T cells (CD3/CD8), helper T cells (CD3/CD4), B cells (CD19), and NK cells (CD49). Control samples were labeled with BP488-conjugated rabbit isotype control (Cell Signaling Technologies #3900S).

Results

Result
Pass (The antibody worked under these conditions)