Anti-BKbeta2 K+ channel [N53/32R] in Western Blot (Rat)

Hybridoma

Data Submitted By: James Trimmer
Lab Name: UC Davis/NIH NeuroMab Facility
Submission Date: March 20, 2024
Publication Date: October 21, 2024 (modified October 21, 2024)
Abstract: Experimental results for Anti-BKbeta2 K+ channel [N53/32R] (hybridoma-derived) in a western blot assay with a rat brain sample.

Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N53/32 (left) or N52A/42 (right) TC supe. — Caption: Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N53/32 (left) or N52A/42 (right) TC supe.
Image attribution: UC Davis/NIH NeuroMab Facility

Any further use of this image requires permission from the lab who submitted the data.

Related Antibody: Anti-BKbeta2 K+ channel [N53/32R]
Addgene #209598

Materials & Methods

Sample

Species: Rat
Cell / Tissue Type: Brain

Primary Antibody

Name: Anti-BKbeta2 K+ channel [N53/32R]
Species: Mouse
Gene / Target: Kcnmb2

Additional Information

Additional Information: NeuroMab hybridoma clone datasheet (Link opens in a new window)
Protocol: Trimmer lab immunoblot labeling protocol (Link opens in a new window)

Results

Result: Pass (The antibody worked under these conditions)