Anti-SynCAM1 [L45/30R] in Western Blot (Rat)
Addgene #209587
- Data Submitted By
- James Trimmer
- Lab Name
- James Trimmer, UC Davis
- Submission Date
- June 17, 2024
- Publication Date
- July 30, 2024 (modified August 21, 2024)
- Abstract
- Experimental results for Anti-SynCAM1 [L45/30R] (Addgene #209587) in a western blot assay with a rat brain lysate. The staining of the Anti-SynCAM1 [L45/30R] recombinant antibody was compared to its parental hybridoma.
Image attribution: James Trimmer, UC Davis
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-SynCAM1 [L45/30R]
Addgene #209587 -
Goat anti-mouse IgG2a
Jackson ImmunoResearch Labs #115-035-206
Materials & Methods
Sample
- Target Species
- Rat
- Cell / Tissue Type
- Brain
- Additional Information
- Rat brain lysates were boiled at 95 °C 5 min prior to loading.
Buffer
- Name
- TBS
- pH
- 7.5
Blocking
- Agent
- Milk
- Concentration
- 4% w/v
- Time
- 45 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-SynCAM1 [L45/30R]
- Source
- Addgene
- Catalog Number
- 209587
- Host Species
- Mouse
- Concentration
- 1 µg/mL
- Time
- 45 min
- Temperature
- Room Temperature
Secondary Antibody
- Name
- Goat anti-mouse IgG2a
- Source
- Jackson ImmunoResearch Labs
- Catalog Number
- 115-035-206
- Concentration
- 1:8333
- Conjugate
- HRP
- Time
- 45 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
- Total rat brain extract was reduced and separated by denaturing SDS-PAGE.
- Protocol
- Trimmer lab immunoblot labeling protocol
- Detection Method
- ECL
- Predicted Molecular Weight
- 50 kDa
Results
- Result
- Pass (The antibody worked under these conditions)
- Describe Results
- Equivalent signal strength and labeling pattern between parental monoclonal antibody and recombinant antibody. The band is running higher than expected at ~75 kDa.