Anti-Botch [N116/14R] in Western Blot (Rat)

Hybridoma

Data Submitted By: James Trimmer
Lab Name: UC Davis/NIH NeuroMab Facility
Submission Date: February 06, 2024
Publication Date: February 06, 2024 (modified March 18, 2024)
Abstract: Experimental results for Anti-Botch [N116/14R] (hybridoma-derived) in a western blot assay with a rat sample.

Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Myctagged Botch1 and untagged Kv2.1 plasmids and probed with N116/14 (left panel) and K89/34 (right panel) TC supe. — Caption: Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Myctagged Botch1 and untagged Kv2.1 plasmids and probed with N116/14 (left panel) and K89/34 (right panel) TC supe.
Image attribution: UC Davis/NIH NeuroMab Facility

Any further use of this image requires permission from the lab who submitted the data.

Related Antibody: Anti-Botch [N116/14R]
Addgene #191818

Materials & Methods

Sample

Species: Rat
Cell / Tissue Type: Brain

Primary Antibody

Name: Anti-Botch [N116/14R]
Species: Mouse
Gene / Target: Chac1

Additional Information

Additional Information: NeuroMab hybridoma clone datasheet (Link opens in a new window)

Results

Result: Fail (The antibody did not work under these conditions)