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Anti-GFP [N86/8R] in Western Blot (Human)

Addgene #184171

Data Submitted By: Meghan Rego
Lab Name: Addgene
Submission Date: February 06, 2024
Publication Date: February 06, 2024 (modified February 06, 2024)
Abstract: Experimental results for Anti-GFP [N86/8R] (Addgene Antibody 184171) in a western blot assay with a human sample.

HEK293 cells were left untransfected or transfected with plasmid 18662, mEGFP-HRas. Whole-cell lysates were prepared and immunoblotted against Anti-GFP N86_8R or Anti-Vinculin as a loading control. — Caption: HEK293 cells were left untransfected or transfected with plasmid 18662, mEGFP-HRas. Whole-cell lysates were prepared and immunoblotted against Anti-GFP N86_8R or Anti-Vinculin as a loading control.
Image attribution: Addgene

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibodies Used: Anti-GFP [N86/8R]
Addgene #184171; goat anti-mouse IgG (H+L) HRP
Thermo Fisher #A16078

Materials & Methods

Sample

Species: Human
Cell / Tissue Type: HEK293

Buffer

Name: TBS-T
pH: 7.4

Blocking

Agent: Milk
Concentration: 5%
Time: 30 min
Temperature: Room Temperature

Primary Antibody

Name: Anti-GFP [N86/8R]
Source: Addgene
Catalog Number: 184171
Species: Mouse
Concentration: 2 µg/mL
Time: 1 hour
Temperature: Room Temperature

View in Addgene Catalog

Secondary Antibody

Name: goat anti-mouse IgG (H+L) HRP
Source: Thermo Fisher
Catalog Number: A16078
Time: 45 min
Temperature: Room Temperature

Additional Information

Additional Information: Whole cell lysates were reduced and separated by denaturing SDS-PAGE
Protocol: Addgene Western Blot protocol
Predicted Molecular Weight: 49 kDa

Results

Result: Pass (The antibody worked under these conditions)