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Anti-Contactin/F3 [K73/20R] in Western Blot (Rat)

Addgene #183003

Data Submitted By: Meghan Rego
Lab Name: Addgene
Submission Date: February 06, 2024
Publication Date: February 06, 2024 (modified February 06, 2024)
Abstract: Experimental results for Anti-Contactin/F3 [K73/20R] (Addgene Antibody 183003) in a western blot assay with a rat sample.

Crude rat membranes immunoblotted against Anti-Contactin/F3 purified recombinant antibody produced at Addgene (R) or hybridoma-derived antibody from the Trimmer Lab at UC Davis (H). Blots were stripped and reprobed with anti-vinculin as a loading control. — Caption: Crude rat membranes immunoblotted against Anti-Contactin/F3 purified recombinant antibody produced at Addgene (R) or hybridoma-derived antibody from the Trimmer Lab at UC Davis (H). Blots were stripped and reprobed with anti-vinculin as a loading control.
Image attribution: Addgene

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibodies Used: Anti-Contactin/F3 [K73/20R]
Addgene #183003; goat anti-mouse IgG (H+L) HRP
Thermo Fisher #A16078

Materials & Methods

Sample

Species: Rat
Cell / Tissue Type: Brain
Total Amount: 40 µg

Buffer

Name: TBS-T
pH: 7.4

Blocking

Agent: Milk
Concentration: 5%
Time: 45 min
Temperature: Room Temperature

Primary Antibody

Name: Anti-Contactin/F3 [K73/20R]
Source: Addgene
Catalog Number: 183003
Species: Mouse
Gene / Target: Cntn1
Concentration: 10 µg/mL
Time: overnight
Temperature: 4 °C

View in Addgene Catalog

Secondary Antibody

Name: goat anti-mouse IgG (H+L) HRP
Source: Thermo Fisher
Catalog Number: A16078
Time: 45 min
Temperature: Room Temperature

Additional Information

Additional Information
Protocol: Addgene Western Blot protocol
Predicted Molecular Weight: 114 kDa

Results

Result: Pass (The antibody worked under these conditions)