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Anti-Pan-FHF-A [N235/22R] in Immunohistochemistry (Mouse)

Addgene #190888

Data Submitted By
Zhuhao Wu
Lab Name
Zhuhao Wu, Weill Cornell Medicine
Submission Date
February 06, 2024
Publication Date
February 06, 2024 (modified February 06, 2024)
Abstract
Experimental results for Anti-Pan-FHF-A [N235/22R] (Addgene Antibody 190888) in an immunohistochemistry assay with a mouse sample.
Caption: Delipidated mouse brain tissue was stained with Anti-Pan-FHF-A [N235/22R].
Image attribution: Zhuhao Wu, Weill Cornell Medicine

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibodies Used
Anti-Pan-FHF-A [N235/22R]
Addgene #190888
HRP-conjugated AffiniPure Goat Anti-Mouse IgG, Fc_ subclass 2a specific
Jackson Labs #115-035-206

Materials & Methods

Sample

Species
Mouse
Cell / Tissue Type
70 µm mounted sagittal section

Fixative

Fixative
Formaldehyde/Paraformaldehyde

Permeabilization / Delipidation

Delipidation
Yes

Blocking

Agent
B1n
Time
3x 1 hour
Temperature
Room Temperature

Primary Antibody

Name
Anti-Pan-FHF-A [N235/22R]
Source
Addgene
Catalog Number
190888
Species
Mouse
Gene / Target
FGF13
Concentration
1 µg/mL
Time
overnight
Temperature
Room Temperature
View in Addgene Catalog

Secondary Antibody

Name
HRP-conjugated AffiniPure Goat Anti-Mouse IgG, Fc_ subclass 2a specific
Source
Jackson Labs
Catalog Number
115-035-206

Additional Information

Additional Information

Tissue type: 70 µm mounted sagittal section

Antigen-retrieval: No

Protocol
Wu lab thick-section immunohistochemistry protocols (Link opens in a new window)

Results

Result
Pass (The antibody worked under these conditions)