Anti-Kv1.6 K+ channel [K19/36R] in Immunohistochemistry (Rat)
Addgene #184211
- Data Submitted By
- James Trimmer
- Lab Name
- James Trimmer, UC Davis
- Submission Date
- October 09, 2024
- Publication Date
- October 18, 2024 (modified November 21, 2024)
- Abstract
- Experimental results for Anti-Kv1.6 K+ channel [K19/36R] (Addgene #184211) in an immunohistochemistry assay with rat brain samples.
![Free-floating rat brain sections were fixed and labeled with purified, recombinant Anti-Kv1.6 K+ channel [K19/36R] or TC Supernatant from NeuroMab clone K19/36. The labeling patterns were compared between the two antibodies.](https://datahub-media.addgene.org/report-images/hv9ri2/K19_36R.png)
Image attribution: James Trimmer, UC Davis
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-Kv1.6 K+ channel [K19/36R]
Addgene #184211 -
Mix of biotinylated goat anti-mouse IgG2a
Jackson ImmunoResearch Labs #115-065-205, 115-065-206, 115-065-207
Materials & Methods
Sample
- Species
- Rat
- Cell / Tissue Type
- Brain
- Preparation
- Free-floating
Buffer
- Name
- PBS
- pH
- 7.4
Fixative
- Fixative
- Formaldehyde/Paraformaldehyde
Permeabilization / Delipidation
- Agent
- PBS containing 1:2000 avidin and 0.5% Triton X-100
- Time
- 45 min
- Temperature
- Room Temperature
Blocking
- Agent
- Serum
- Concentration
- 0.5% w/v
- Time
- 45 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-Kv1.6 K+ channel [K19/36R]
- Source
- Addgene
- Catalog Number
- 184211
- Species
- Mouse
- Gene / Target
- Kcna6
- Concentration
- 1 µg/mL
- Time
- Overnight
- Temperature
- 4 ˚C
Secondary Antibody
- Name
- Mix of biotinylated goat anti-mouse IgG2a
- Source
- Jackson ImmunoResearch Labs
- Catalog Number
- 115-065-205, 115-065-206, 115-065-207
- Concentration
- 1 µg/mL
- Time
- 60 min
- Temperature
- Room Temperature
Additional Information
- Protocol
- Trimmer lab immunohistochemistry protocol (Link opens in a new window)
- Controls
- The labeling pattern was compared to the NeuroMab K19/36.
Results
- Result
- Pass (The antibody worked under these conditions)
- Describe Results
- Labeling pattern was compared and found to be equivalent to the NeuroMab K19/36.