Anti-Integrin alpha M [IPI-M1/70] in Flow Cytometry (Human)
Addgene #213665
- Data Submitted By
- IPI Staff
- Lab Name
- Institute for Protein Innovation (IPI)
- Submission Date
- March 20, 2024
- Publication Date
- March 25, 2024 (modified March 25, 2024)
- Abstract
- Experimental results for Anti-Integrin alpha M [IPI-M1/70] (Addgene Antibody 213665) in a flow cytometry assay with a human sample.
![Flow cytometry data showing positive labeling by Anti-Integrin alpha M [IPI-M1/70] antibody (#213665) in human CD8+ T cells, CD4+ T cells, B cells, and NK cells. Human peripheral blood mononuclear cells (PBMCs) were isolated from whole blood samples. These cells were labeled with Anti-Integrin alpha M [IPI-M1/70] antibody conjugated with BroadPharma Fluor 488 NHS Ester (BP488). Control samples were labeled with BP488-conjugated rabbit isotype control (Cell Signaling Technologies #3900S). The cells were also labeled with cell type markers to distinguish leukocytes (CD45), T cells (CD3), cytotoxic T cells (CD3/CD8), helper T cells (CD3/CD4), B cells (CD19), and NK cells (CD49). After labeling and washing, flow cytometry experiments were performed on a CytoFLEX flow cytometer.](https://datahub-media.addgene.org/report-images/hpfjyx/213665-Flow_Cytometry-2024-02-03_203420.091537.png)
Image attribution: Institute for Protein Innovation (IPI)
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibody Used
-
Anti-Integrin alpha M [IPI-M1/70]
Addgene #213665
Materials & Methods
Sample
- Target Species
- Human
- Cell / Tissue Type
- Peripheral blood mononuclear cells (PBMC)
Blocking
- Agent
- 1% BSA with Fc blocker
- Time
- 20 min
- Temperature
- 4 ˚C
Primary Antibody
- Name
- Anti-Integrin alpha M [IPI-M1/70]
- Source
- Addgene
- Catalog Number
- 213665
- Host Species
- Rabbit
- Target Antigen
- Itgam
- Concentration
- 10 µg/mL
- Conjugate
- BroadPharma Fluor 488 NHS Ester (BP488)
- Time
- 30 min
- Temperature
- 4 ˚C
Additional Information
- Additional Information
Human PBMCs were isolated from whole blood sample using a Cell Preparation Tube (CPT) with sodium heparin and resuspended in Dulbecco's Phosphate Buffered Saline (DPBS)-based blocking buffer.
Detection: Flow cytometry experiments were performed on a CytoFLEX flow cytometer. The cells were also labeled with cell type markers to distinguish leukocytes (CD45), T cells (CD3), cytotoxic T cells (CD3/CD8), helper T cells (CD3/CD4), B cells (CD19), and NK cells (CD49). Control samples were labeled with BP488-conjugated rabbit isotype control (Cell Signaling Technologies #3900S).
Results
- Result
- Pass (The antibody worked under these conditions)