Anti-Neurexin-2-alpha [IPI-mNRXN2a.10] in Immunohistochemistry (mouse)
Addgene #237806
- Data Submitted By
- Karl Murray
- Lab Name
- NIH NeuroMab, UC Davis
- Submission Date
- March 03, 2026
- Publication Date
- March 23, 2026 (modified March 23, 2026)
- DOI
- https://doi.org/10.57733/addgene.hnmfin
- Abstract
- Experimental results for Anti-Neurexin-2-alpha [IPI-mNRXN2a.10] (Addgene#237806) in an immunohistochemistry assay with a mouse sample.
- Citation
-
Anti-Neurexin-2-alpha [IPI-mNRXN2a.10] in Immunohistochemistry (mouse). Murray K 2026. Addgene Report, https://doi.org/10.57733/addgene.hnmfin
![Free-floating mouse brain sections were fixed and labeled with Anti-Neurexin-2-alpha [IPI-mNRXN2a.10]. Hippocampal labeling is shown. Abbreviations: DG: dentate gyrus; CA3: cornu ammonis 3; CA2: cornu ammonis 2; CA1: cornu ammonis 1; sg: dentate gyrus stratum granulosum; ml: molecular layer; sp: stratum pyramidale; sr: stratum radiatum; so: stratum oriens.](https://datahub-media.addgene.org/report-images/hnmfin/NRXN2a.10_NeuroMab_IHC.png)
Image attribution: NIH NeuroMab
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-Neurexin-2-alpha [IPI-mNRXN2a.10]
Addgene #237806 -
Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated
Vector Laboratories #BA-1000-1
Materials & Methods
Sample
- Target Species
- Mouse
- Cell / Tissue Type
- Brain
- Additional Information
- 30 µm section
- Preparation
- Free-floating
Buffer
- Name
- TBS (0.15 M NaCl, 50 mM Tris)
- pH
- 7.5
Fixative
- Fixative
- Paraformaldehyde, 4%
Permeabilization / Delipidation
- Agent
- TBS with 0.5% Triton X-100, 0.01 mg/mL Avidin
- Concentration
- 45 min
- Temperature
- 4 ˚C
Blocking
- Agent
- TBS with 5% normal goat serum, 0.1% Triton X-100, 0.01875 mg/mL Biotin
- Time
- 45 min
- Temperature
- 4 ˚C
Primary Antibody
- Name
- Anti-Neurexin-2-alpha [IPI-mNRXN2a.10]
- Source
- Addgene
- Catalog Number
- 237806
- Host Species
- Rabbit
- Target Antigen
- NRXN2a
- Concentration
- 5 µg/mL (1:200 dilution)
- Time
- Overnight
- Temperature
- 4 ˚C
Secondary Antibody
- Name
- Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated
- Source
- Vector Laboratories
- Catalog Number
- BA-1000-1
- Concentration
- 0.5 µg/mL
- Time
- 60 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
Previous studies show NRXN2-mediated signaling is particularly relevant in the CA1 region and the dentate gyrus within the hippocampus, where its deletion increases immature dendritic spines (Lin et al., 2023).
References
Lin et al. (2023). Neurexin-2: An inhibitory neurexin that restricts excitatory synapse formation in the hippocampus. Science Advances, 9(1): eadd8856. https://doi.org/10.1126/sciadv.add8856 (Link opens in a new window) PMID: 36608123 (Link opens in a new window)
- Protocol
- Trimmer lab immunohistochemistry protocol (Link opens in a new window)
Results
- Result
- Pass (The antibody worked under these conditions)
- Describe Results
- Strong signal for NRXN2