ICC/IF for Anti-IGDCC3 (PUNC) [IPI-IGDC3.27]
Addgene #241896
- Data Submitted By
- Alexander Jaworski
- Lab Name
- Alexander Jaworski, Brown University
- Submission Date
- June 06, 2024
- Publication Date
- March 06, 2026 (modified March 06, 2026)
- DOI
- https://doi.org/10.57733/addgene.h6kmyi
- Abstract
- Collagen gel COS-7 cell cultures labeled with Anti-IGDCC3 (PUNC) [IPI-IGDC3.27] (Addgene #241896), Anti-GFP, and Anti-Hoechst.
- Citation
-
ICC/IF for Anti-IGDCC3 (PUNC) [IPI-IGDC3.27]. Jaworski A 2026. Addgene Report, https://doi.org/10.57733/addgene.h6kmyi
![Collagen gel COS-7 cell cultures labeled with Anti-IGDCC3 (PUNC) [IPI-IGDC3.27], Anti-GFP, and Anti-Hoechst.](https://datahub-media.addgene.org/report-images/h6kmyi/IPI-IGDC3.37_IF1.png)
Image attribution: Alexander Jaworski, Brown University
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-IGDCC3 (PUNC) [IPI-IGDC3.27]
Addgene #241896 -
Alexa 647-conjugated donkey anti-rabbit
Invitrogen #A-31573
Materials & Methods
Sample
- Target Species
- Human
- Cell / Tissue Type
- COS-7 cells
- Additional Information
- COS-7 cells were co-transfected with a human IGDCC3 (PUNC) expression plasmid and a GFP expression plasmid as a transfection control.
Primary Antibody
- Name
- Anti-IGDCC3 (PUNC) [IPI-IGDC3.27]
- Source
- Addgene
- Catalog Number
- 241896
- Host Species
- Rabbit
- Target Antigen
- IGDCC3
- Concentration
- 1:200
Secondary Antibody
- Name
- Alexa 647-conjugated donkey anti-rabbit
- Source
- Invitrogen
- Catalog Number
- A-31573
- Concentration
- 1:200 of 2 mg/mL stock
Additional Information
- Additional Information
Primary antibodies used were chick polyclonal antibodies against GFP (Abcam, 1:200) and Anti-IGDCC3 (PUNC) [IPI-IGDC3.27] (Addgene, 1:200).
Secondary antibodies were Alexa 647-conjugated donkey anti-rabbit (Invitrogen, A-31573) at a 1:200 dilution of a 2 mg/mL stock for the detection of Anti-IGDCC3 (PUNC) [IPI-IGDC3.27] and Alexa 647-conjugated donkey anti-chicken (Jackson ImmunoResearch Laboratories, 703-605-155) at a 1:200 dilution of a 1.5 mg/mL stock for the detection of the chicken Anti-GFP. Hoechst 33342 (Molecular Probes, 1:1000) was added with the secondary antibodies. Images were acquired on a Nikon Ti-E microscope or Nikon CSU-W1 SoRa Ti2-E microscope.
Results
- Result
- Pass (The antibody worked under these conditions)
- Describe Results
- Positive labeling for IGDCC3