Anti-LPL [5D2] in Western Blot (Human)
Addgene #211752
- Data Submitted By
- Meghan Rego
- Lab Name
- Addgene
- Submission Date
- May 08, 2024
- Publication Date
- May 31, 2024 (modified June 07, 2024)
- Abstract
- Experimental results for Anti-LPL [5D2] (Addgene #211752) in a western blot assay with a human sample.
Image attribution: Meghan Rego
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-LPL [5D2]
Addgene #211752 -
Goat anti-mouse IgG (H+L) HRP
Thermo Fisher #A16078
Materials & Methods
Sample
- Species
- Human
- Cell / Tissue Type
- HEK293
- Additional Information
- HEK293 cell lysates expressing B2M-DDK (Catalog number LC401311) or LPL-DDK (Catalog number LC400089) were obtained from Origene.
- Total Amount
- 10 µg
Buffer
- Name
- TBS-T
- pH
- 7.4
Blocking
- Agent
- BSA
- Concentration
- 5%
- Time
- 60 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-LPL [5D2]
- Source
- Addgene
- Catalog Number
- 211752
- Species
- Mouse
- Gene / Target
- LPL
- Concentration
- 4 µg/mL
- Time
- Overnight
- Temperature
- 4 ˚C
Secondary Antibody
- Name
- Goat anti-mouse IgG (H+L) HRP
- Source
- Thermo Fisher
- Catalog Number
- A16078
- Concentration
- 0.4 µg/mL
- Conjugate
- HRP
- Time
- 45 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
- HEK293 cell lysates expressing B2M-DDK or LPL-DDK were immunoblotted against anti-LPL (Addgene #211752), anti-DYKDDDK (Addgene #194502), or anti-Vinculin (Enzo, catalog number BMLVG61100100) as a loading control.
- Protocol
- Addgene Western Blot protocol
- Detection Method
- ECL
- Predicted Molecular Weight
- 53.2 kDa
Results
- Result
- Pass (The antibody worked under these conditions)