Anti-Pan-QKI [N147/6.1R] in Western Blot (Rat)

Hybridoma

Data Submitted By: James Trimmer
Lab Name: UC Davis/NIH NeuroMab Facility
Submission Date: February 06, 2024
Publication Date: February 06, 2024 (modified March 18, 2024)
Abstract: Experimental results for Anti-Pan-QKI [N147/6.1R] (hybridoma-derived) in a western blot assay with a rat sample.

Immunoblots on membranes prepared from whole mouse (MBM) and rat (RBM) brain, and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)], and cerebellum [HBM(Cb)]. — Caption: Immunoblots on membranes prepared from whole mouse (MBM) and rat (RBM) brain, and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)], and cerebellum [HBM(Cb)].

Adult rat brain immunoblot. — Caption: Adult rat brain immunoblot.

Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Flag-tagged QKI-5, QKI-6, QKI-7 and QKI-7b or untagged Kv2.1 plasmids and probed with N147/6 (left) and mouse anti-Flag (right). — Caption: Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with Flag-tagged QKI-5, QKI-6, QKI-7 and QKI-7b or untagged Kv2.1 plasmids and probed with N147/6 (left) and mouse anti-Flag (right).

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Related Antibody: Anti-Pan-QKI [N147/6.1R]
Addgene #180114

Materials & Methods

Sample

Species: Rat
Cell / Tissue Type: Brain membrane fractions

Primary Antibody

Name: Anti-Pan-QKI [N147/6.1R]
Species: Mouse
Gene / Target: QKI

Additional Information

Additional Information: NeuroMab hybridoma clone datasheet (Link opens in a new window)

Results

Result: Pass (The antibody worked under these conditions)