ICC/IF for Anti-Slit-1 [IPI-SLIT1.75]
Addgene #259424
- Data Submitted By
- Ana Saintpierre and Homaira Nawabi
- Lab Name
- Homaira Nawabi, Inserm
- Submission Date
- July 27, 2026
- Publication Date
- August 05, 2026 (modified August 14, 2026)
- DOI
- https://doi.org/10.57733/addgene.dckoln
- Abstract
- Experimental results for Anti-Slit-1 [IPI-SLIT1.75] (Addgene #259424) in an immunofluorescence (IF) binding assay with COS cells expressing human Slit1, Slit2, or Slit3.
- Citation
-
ICC/IF for Anti-Slit-1 [IPI-SLIT1.75]. Saintpierre A, Nawabi H 2026. Addgene Report, https://doi.org/10.57733/addgene.dckoln
![A) Immunofluorescence (IF) of COS cells transfected with Slit-1, Slit-2, or Slit-3 and labeled with Anti-Slit-1 [IPI-SLIT1.75] (Addgene #259424). Anti-Myc labeling was used to demonstrate successful transfection (shown only for Slit-2).](https://datahub-media.addgene.org/report-images/dckoln/SLIT1.75_Nawabi_IF.png)
Image attribution: Homaira Nawabi, Inserm
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-Slit-1 [IPI-SLIT1.75 ]
Addgene #259424 -
Alexa Fluor 647 F(ab')2 goat anti-rabbit IgG Fc fragment
Jackson ImmunoResearch #111-606-046
Materials & Methods
Sample
- Target Species
- Human
- Cell / Tissue Type
- COS (African green monkey kidney cell line)
- Additional Information
- Cos cells were seeded on coverslips and when 70% of confluence was reached, cells were transfected for 48 h with plasmids expressing Myc-tagged Slit1, Slit2, or Slit3 (3 µg of DNA), using PEI transfection reagent.
Buffer
- Name
- PBS
Fixative
- Fixative
- Formaldehyde/Paraformaldehyde
- Time
- 15 min
- Temperature
- Room Temperature
Permeabilization
- Agent
- Triton X-100 in PBS
- Concentration
- 0.10%
- Time
- 10 min
- Temperature
- Room Temperature
Blocking
- Agent
- Normal goat serum + BSA
- Concentration
- 5% serum, 5% BSA
- Time
- 30 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-Slit-1 [IPI-SLIT1.75 ]
- Source
- Addgene
- Catalog Number
- 259424
- Host Species
- Rabbit
- Target Antigen
- Slit-1
- Concentration
- 2 µg/mL (1:500 dilution)
- Time
- 120 min
- Temperature
- Room Temperature
Secondary Antibody
- Name
- Alexa Fluor 647 F(ab')2 goat anti-rabbit IgG Fc fragment
- Source
- Jackson ImmunoResearch
- Catalog Number
- 111-606-046
- Concentration
- 2 µg/mL
- Time
- 60 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
Controls
Positive control: Anti-Myc tag (data for Slit-1-Myc and Slit-3-Myc not shown).
Negative control: No transfection.
Results
- Result
- Pass (The antibody worked under these conditions)
- Describe Results
- Positive for Slit-1 labeling. Negative for Slit-2 or Slit-3 labeling.