Anti-Glypican 4 (GPC4) [IPI-mGPC4.9] in Immunohistochemistry (Rat)
Addgene #222516
- Data Submitted By
- James Trimmer
- Lab Name
- James Trimmer, UC Davis
- Submission Date
- August 26, 2025
- Publication Date
- October 30, 2025 (modified October 31, 2025)
- DOI
- https://doi.org/10.57733/addgene.cul4li
- Abstract
- Experimental results for Anti-Glypican 4 (GPC4) [IPI-mGPC4.9] (Addgene #222516) in an immunohistochemistry assay with a rat sample.
- Citation
-
Anti-Glypican 4 (GPC4) [IPI-mGPC4.9] in Immunohistochemistry (Rat). Trimmer J 2025. Addgene Report, https://doi.org/10.57733/addgene.cul4li
![Free-floating rat brain sections (30 µm) were fixed and labeled with purified Anti-Glypican 4 (GPC4) [IPI-mGPC4.9] (Addgene #222516).](https://datahub-media.addgene.org/report-images/cul4li/mGPC4.9_IHC.png)
Image attribution: James Trimmer, UC Davis
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-Glypican 4 (GPC4) [IPI-mGPC4.9]
Addgene #222516 -
Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated
Vector Laboratories #BA-1000-1
Materials & Methods
Sample
- Target Species
- Rat
- Cell / Tissue Type
- Brain
- Additional Information
- 30 µm section
- Preparation
- Free-floating
Buffer
- Name
- TBS (0.15 M NaCl, 50 mM Tris)
- pH
- pH 7.5
Fixative
- Fixative
- Formaldehyde/Paraformaldehyde
Permeabilization / Delipidation
- Agent
- TBS with 0.5% Triton X-100, 0.01 mg/mL Avidin
- Time
- 45 min
- Temperature
- 4 ˚C
Blocking
- Agent
- TBS with 5% normal goat serum, 0.1% Triton X-100, 0.01875 mg/mL Biotin
- Time
- 45 min
- Temperature
- 4 ˚C
Primary Antibody
- Name
- Anti-Glypican 4 (GPC4) [IPI-mGPC4.9]
- Source
- Addgene
- Catalog Number
- 222516
- Host Species
- Rabbit
- Target Antigen
- GPC4
- Concentration
- 5 µg/mL
- Time
- Overnight
- Temperature
- 4 ˚C
Secondary Antibody
- Name
- Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated
- Source
- Vector Laboratories
- Catalog Number
- BA-1000-1
- Concentration
- 0.5 µg/mL
- Time
- 60 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
Validated by comparing to expression levels from in situ hybridization done by Allen Brain Atlas (Link opens in a new window) from a sagittal section of P56 mouse brain.
- Protocol
Trimmer lab immunohistochemistry protocol (Link opens in a new window)
Results
- Result
- Pass (The antibody worked under these conditions)
- Describe Results
- Recognizes GPC4. Weak signal.