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Anti-Neuroligin-3 [N110/29.4R] in Western Blot (Mouse)

Hybridoma

Data Submitted By
James Trimmer
Lab Name
UC Davis/NIH NeuroMab Facility
Submission Date
February 06, 2024
Publication Date
February 06, 2024 (modified March 18, 2024)
Abstract
Experimental results for Anti-Neuroligin-3 [N110/29.4R] (hybridoma-derived) in a western blot assay with a mouse sample.
Adult mouse brain immunoblot: extracts of brains from Neuroligin-3 wild-type (WT) and knockout (KO) mice were probed with N110/29 TC supe. Data courtesy of Marc Bolliger and Tom Sudhof, UTSW (now at Stanford).
Caption: Adult mouse brain immunoblot: extracts of brains from Neuroligin-3 wild-type (WT) and knockout (KO) mice were probed with N110/29 TC supe. Data courtesy of Marc Bolliger and Tom Sudhof, UTSW (now at Stanford).
Adult mouse brain membrane (MBM) and transfected cell immunoblot: extracts of MBM and COS cells transiently transfected with Neuroligin-1, -2, -3, -4 and -4* plasmids and probed with N110/29 TC supe. Data courtesy of Marc Bolliger and Tom Sudhof, UTSW (now at Stanford).
Caption: Adult mouse brain membrane (MBM) and transfected cell immunoblot: extracts of MBM and COS cells transiently transfected with Neuroligin-1, -2, -3, -4 and -4* plasmids and probed with N110/29 TC supe. Data courtesy of Marc Bolliger and Tom Sudhof, UTSW (now at Stanford).

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Related Antibody
Anti-Neuroligin-3 [N110/29.4R]
Addgene #180120

Materials & Methods

Sample

Species
Mouse
Cell / Tissue Type
Brain extracts and membranes

Primary Antibody

Name
Anti-Neuroligin-3 [N110/29.4R]
Species
Mouse
Gene / Target
Nlgn3

Additional Information

Additional Information

NeuroMab hybridoma clone datasheet

Results

Result
Pass (The antibody worked under these conditions)
Knockout Validation
Yes