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Anti-Slit-1 [IPI-SLIT1.80] in Surface Plasmon Resonance (SPR)

Addgene #259425

Data Submitted By
Murali Anuganti , Aditi Kachare , Travis Riedel and Deborah Moshinsky
Lab Name
Institute for Protein Innovation (IPI)
Submission Date
July 27, 2026
Publication Date
August 05, 2026 (modified August 14, 2026)
DOI
https://doi.org/10.57733/addgene.940ms0
Abstract
Experimental results for Anti-Slit-1 [IPI-SLIT1.80] (Addgene #259425) in SPR assay with a human sample.
Citation
Anti-Slit-1 [IPI-SLIT1.80] in Surface Plasmon Resonance (SPR). Anuganti M, Kachare A, Riedel T, Moshinsky D 2026. Addgene Report, https://doi.org/10.57733/addgene.940ms0
Surface Plasmon Resonance (SPR) kinetics analysis of the interaction between Anti-Slit-1 [IPI-SLIT1.80] (Addgene #259425) and human Slit-1. SPR binding kinetics were measured on a Carterra LSA using HC30M chips (Carterra, cat. #4279) at 25 °C. Goat anti-rabbit IgG Fc (Jackson ImmunoResearch #111-005-046) was immobilized via amine coupling, and test antibodies were captured using a 96-channel print-head. Antigens (400 nM to five lower concentrations, 2-fold dilutions) were injected in antigen buffer (20 mM HEPES pH 7.4, 150 mM NaCl, 1 mM CaCl₂, 1 mM MgCl₂, 0.005% Tween 80) with 300 s association/ dissociation phases and acid regeneration. Data (reference/buffer subtracted, smoothed) were globally fit to a 1:1 Langmuir model to derive ka, kd, and KD using Carterra Kinetics software v1.9.2.44.63, and replotted in OriginPro 2023b. Results show a high-affinity and specific binding event between the antibody and antigen. (Right) IPI-Slit1.80 was originally generated against the hNSlit1 antigen. Cross-reactivity assessment was performed using antibody samples against mSlit3C, hNSlit3, and hSlit2C andantigens. No binding was observed to any of the tested Slit2 or Slit3 constructs, demonstrating that IPI-Slit1.80 is highly specific for the hNSlit1 antigen. As shown in the SPR data below, all tested Slit2 and Slit3 antigens exhibited flat sensorgram responses, indicating a lack of detectable interaction.
Caption: Surface Plasmon Resonance (SPR) kinetics analysis of the interaction between Anti-Slit-1 [IPI-SLIT1.80] (Addgene #259425) and human Slit-1. SPR binding kinetics were measured on a Carterra LSA using HC30M chips (Carterra, cat. #4279) at 25 °C. Goat anti-rabbit IgG Fc (Jackson ImmunoResearch #111-005-046) was immobilized via amine coupling, and test antibodies were captured using a 96-channel print-head. Antigens (400 nM to five lower concentrations, 2-fold dilutions) were injected in antigen buffer (20 mM HEPES pH 7.4, 150 mM NaCl, 1 mM CaCl₂, 1 mM MgCl₂, 0.005% Tween 80) with 300 s association/ dissociation phases and acid regeneration. Data (reference/buffer subtracted, smoothed) were globally fit to a 1:1 Langmuir model to derive ka, kd, and KD using Carterra Kinetics software v1.9.2.44.63, and replotted in OriginPro 2023b. Results show a high-affinity and specific binding event between the antibody and antigen. (Right) IPI-Slit1.80 was originally generated against the hNSlit1 antigen. Cross-reactivity assessment was performed using antibody samples against mSlit3C, hNSlit3, and hSlit2C andantigens. No binding was observed to any of the tested Slit2 or Slit3 constructs, demonstrating that IPI-Slit1.80 is highly specific for the hNSlit1 antigen. As shown in the SPR data below, all tested Slit2 and Slit3 antigens exhibited flat sensorgram responses, indicating a lack of detectable interaction.
Image attribution: Institute for Protein Innovation (IPI)

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibody Used
Anti-Slit-1 [IPI-SLIT1.80]
Addgene #259425

Materials & Methods

Sample

Target Species
Human

Primary Antibody

Name
Anti-Slit-1 [IPI-SLIT1.80]
Source
Addgene
Catalog Number
259425
Host Species
Rabbit
Target Antigen
Slit-1
Concentration
4 µg/mL (1:250 dilution)
Temperature
Room Temperature
View in Addgene Catalog

Results

Result
Pass (The antibody worked under these conditions)
Describe Results
Results show a high-affinity and specific binding event between the antibody and antigen. Binding for Slit2 and Slit3 was not observed.