Anti-TrpC5 [N67/15R] in Western Blot (Mouse)

Hybridoma

Data Submitted By: David Clapham
Lab Name: David Clapham, HHMI/Harvard Medical School
Submission Date: February 06, 2024
Publication Date: February 06, 2024 (modified March 18, 2024)
Abstract: Experimental results for Anti-TrpC5 [N67/15R] (hybridoma-derived) in a western blot assay with a mouse sample.

Immunoprecipitation analysis of TRPC5 protein in brain microsomes extracted from wild-type (WT) and TRPC5 knockout (KO) littermates. — Caption: Immunoprecipitation analysis of TRPC5 protein in brain microsomes extracted from wild-type (WT) and TRPC5 knockout (KO) littermates.
Image attribution: David Clapham, HHMI/Harvard Medical School

Any further use of this image requires permission from the lab who submitted the data.

Related Antibody: Anti-TrpC5 [N67/15R]
Addgene #184205

Materials & Methods

Sample

Species: Mouse
Cell / Tissue Type: Brain

Primary Antibody

Name: Anti-TrpC5 [N67/15R]
Species: Mouse
Gene / Target: TRPC5

Additional Information

Additional Information: NeuroMab hybridoma clone datasheet (Link opens in a new window)
Protocol: Trimmer lab immunoblot labeling protocol (Link opens in a new window)

Results

Result: Pass (The antibody worked under these conditions)
Knockout Validation: Yes