IHC-KO for Anti-CNTN2 (TAG-1) [IPI-CNTN2.57]
Addgene #254655
- Data Submitted By
- Alexander Jaworski
- Lab Name
- Alexander Jaworski, Brown University
- Submission Date
- September 17, 2026
- Publication Date
- September 28, 2026 (modified September 28, 2026)
- DOI
- https://doi.org/10.57733/addgene.8abeqh
- Abstract
- Brachial-level transverse sections of E12.5 wild-type and CNTN2 -/- (CNTN2 KO) mouse embryos were labeled with Anti-CNTN2 (TAG-1) [IPI-CNTN2.57] (Addgene #254655). No labeling is observed on CNTN2 -/- sections.
- Citation
-
IHC-KO for Anti-CNTN2 (TAG-1) [IPI-CNTN2.57]. Jaworski A 2026. Addgene Report, https://doi.org/10.57733/addgene.8abeqh
![Brachial-level transverse sections of E12.5 wild-type and CNTN2 -/- (CNTN2 KO) mouse embryos were labeled with Anti-CNTN2 (TAG-1) [IPI-CNTN2.57] (Addgene #254655). No labeling is observed on CNTN2 -/- sections.](https://datahub-media.addgene.org/report-images/8abeqh/Jaworski_CNTN2_IHCKO.png)
Image attribution: Alexander Jaworski, Brown University
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-CNTN2 (TAG-1) [IPI-CNTN2.57]
Addgene #254655 -
Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594
Invitrogen #A-21207
Materials & Methods
Sample
- Target Species
- Mouse
- Cell / Tissue Type
- Spinal cord
- Additional Information
- 150 μm sections
Primary Antibody
- Name
- Anti-CNTN2 (TAG-1) [IPI-CNTN2.57]
- Source
- Addgene
- Catalog Number
- 254655
- Host Species
- Rabbit
- Target Antigen
- CNTN2
- Concentration
- 1:200 of a 1 mg/mL stock
Secondary Antibody
- Name
- Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 594
- Source
- Invitrogen
- Catalog Number
- A-21207
- Concentration
- 1:200 of 2 mg/mL stock
Additional Information
- Additional Information
All spinal cord sections were collected from brachial level. Immunohistochemistry (IHC) on 150 μm-thick vibratome sections, 2D neural tissue explants, collagen gel COS-7 cell cultures, and Dunn chamber cultures were described previously (Suter et al., 2020), with blocking buffer used for IHC on Dunn chamber cultures, and vibratome sections altered to include 2.5% donkey serum in lieu of bovine serum albumin.
The primary antibody used for IHC was Anti-CNTN2 (TAG-1) [IPI-CNTN2.57] (Addgene #254655, 1:200).
The secondary antibody used was the Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed secondary antibody, Alexa Fluor™ 594 (Invitrogen, A-21207) at a 1:200 dilution of a 2 mg/mL stock for the detection of Anti-CNTN2 (TAG-1) [IPI-CNTN2.57]. Images were acquired on a Nikon Ti-E microscope or Nikon CSU-W1 SoRa Ti2-E microscope.
References
Suter et al. (2020). TAG-1 multifunctionality coordinates neuronal migration, axon guidance, and fasciculation. Cell Rep, 30(4):1164-1177. https://doi.org/10.1016/j.celrep.2019.12.085 (Link opens in a new window) PMID: 31995756 (Link opens in a new window)
Results
- Result
- Pass (The antibody worked under these conditions)
- Describe Results
- Signal for CNTN2 abolished in KO tissue
- Knockout Validation
- Yes