IF for Anti-V5 [IPI-SV5-Pk1]
Addgene #218107
- Data Submitted By
- Jason Aoto
- Lab Name
- Jason Aoto, University of Colorado School of Medicine
- Submission Date
- June 14, 2024
- Publication Date
- July 24, 2025 (modified July 25, 2025)
- DOI
- https://doi.org/10.57733/addgene.83oxaa
- Abstract
- Representative confocal images of surface anti-V5 labeling of wildtype and V5-Nrxn3 neurons in homozygous V5-Nrxn3 knock-in mice (Lloyd et al., 2023 Nat Commun.) using Anti-V5 [IPI-SV5-Pk1] antibody (Addgene #218107).
- Citation
-
IF for Anti-V5 [IPI-SV5-Pk1]. Aoto J 2025. Addgene Report, https://doi.org/10.57733/addgene.83oxaa
![Representative confocal images of surface anti-V5 labeling of wildtype and V5-Nrxn3 neurons in homozygous V5-Nrxn3 knock-in mice (Lloyd et al., 2023 Nat Commun.) using Anti-V5 [IPI-SV5-Pk1].](https://datahub-media.addgene.org/report-images/83oxaa/IF_Anti-V5_IPI-SV5-Pk1_AotoJ.png)
Image attribution: Jason Aoto, PhD
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-V5 [IPI-SV5-Pk1]
Addgene #218107 -
Goat anti-Rabbit AF647
JacksonImmuno #111-605-045
Materials & Methods
Sample
- Species
- Mouse
- Cell / Tissue Type
- Homozygous V5-Nrxn3 knock-in mice
- Additional Information
- See: Lloyd et al., 2023 Nat Commun.
Buffer
- Name
- HEPES ACSF: 140 mM NaCl, 5 mM KCl, 2 mM CaCl2, 10 mM HEPES, 10 mM Glucose
- pH
- 7.4
Fixative
- Fixative
- Formaldehyde/Paraformaldehyde
- Time
- 15 min
- Temperature
- Room Temperature
Blocking
- Agent
- 2% Normal Goat Serum + 0.02% NaAz in PBS
- Time
- 1 h
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-V5 [IPI-SV5-Pk1]
- Source
- Addgene
- Catalog Number
- 218107
- Species
- Rabbit
- Gene / Target
- V5 tag
- Concentration
- 10 µg/mL
- Time
- 20 min at 37 °C
Secondary Antibody
- Name
- Goat anti-Rabbit AF647
- Source
- JacksonImmuno
- Catalog Number
- 111-605-045
- Species
- Goat
- Concentration
- 1:500
- Conjugate
- AlexaFluor 647
- Time
- 1 h
- Temperature
- Room Temperature
Results
- Result
- Pass (The antibody worked under these conditions)