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Anti-6xHis [N144/14R] in Western Blot (Human)

Addgene #184180

Data Submitted By
Meghan Rego
Lab Name
Addgene
Submission Date
February 06, 2024
Publication Date
February 06, 2024 (modified February 06, 2024)
Abstract
Experimental results for Anti-6xHis [N144/14R] (Addgene Antibody 184180) in a western blot assay with a human sample.
Caption: HEK293 cells were left untransfected or transfected with AAV-EF1a-jGCaMP8f-WPRE (+176756). Whole cell lysates were prepared and probed with Anti-6xHis [N144/14R] p8191 and then stripped and reprobed with anti-vinculin as a loading control.
Image attribution: Addgene

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibodies Used
Anti-6xHis [N144/14R]
Addgene #184180
goat anti-mouse IgG (H+L) HRP
Thermo Fisher #A16078

Materials & Methods

Sample

Species
Human
Cell / Tissue Type
HEK293
Total Amount
30 µg

Buffer

Name
TBS-T
pH
7.4

Blocking

Agent
Milk
Concentration
5%
Time
45 min
Temperature
Room Temperature

Primary Antibody

Name
Anti-6xHis [N144/14R]
Source
Addgene
Catalog Number
184180
Species
Mouse
Concentration
2 µg/mL
Time
45 min
Temperature
Room Temperature
View in Addgene Catalog

Secondary Antibody

Name
goat anti-mouse IgG (H+L) HRP
Source
Thermo Fisher
Catalog Number
A16078
Time
45 min
Temperature
Room Temperature

Additional Information

Additional Information
Protocol
Addgene Western Blot protocol
Predicted Molecular Weight
48 kDa

Results

Result
Pass (The antibody worked under these conditions)