Anti-APP [6E10-A5] in Immunohistochemistry (Human)
Addgene #226376
- Data Submitted By
- Zhuhao Wu and Wei Wang
- Lab Name
- Zhuhao Wu, Weill Cornell Medicine
- Submission Date
- November 27, 2024
- Publication Date
- December 03, 2024 (modified December 12, 2024)
- DOI
- https://doi.org/10.57733/addgene.6ncijt
- Abstract
- Experimental results for Anti-APP [6E10-A5] (Addgene #226376) in an immunohistochemistry assay with human brain samples.
- Citation
-
Anti-APP [6E10-A5] in Immunohistochemistry (Human). Wu Z, Wang W 2024. Addgene Report, https://doi.org/10.57733/addgene.6ncijt
![Brain sections were labeled with Anti-APP [6E10-A5] from Addgene (226376-rAb). As expected, the labeling patterns in individuals with Alzheimer's or Hoarding disorder suggests APP expression while that of an individual with diabetes and cancer does not. Human brain tissue was analyzed from postmortem sources and is available thanks to the generosity of the tissue donors and their families through Neuropathology Brain Bank & Research CoRE at Mount Sinai.](https://datahub-media.addgene.org/report-images/6ncijt/6e10a5_human.png)
Image attribution: Zhuhao Wu and Wei Wang, Weill Cornell Medicine
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-APP [6E10-A5]
Addgene #226376 -
HRP-conjugated AffiniPure goat anti-mouse IgG, Fcγ subclass 2a specific
Jackson ImmunoResearch Labs #115-035-206
Materials & Methods
Sample
- Species
- Human
- Cell / Tissue Type
- Brain
- Additional Information
- 70 µm thick section.
- Preparation
- Free-floating
Buffer
- Name
- PBS
Fixative
- Fixative
- Formaldehyde/Paraformaldehyde
Permeabilization / Delipidation
- Delipidation
- Yes
Blocking
- Agent
- PTxwH buffer
Primary Antibody
- Name
- Anti-APP [6E10-A5]
- Source
- Addgene
- Catalog Number
- 226376
- Species
- Mouse
- Gene / Target
- APP
- Concentration
- 1 µg/mL
- Time
- Overnight
- Temperature
- 4 ˚C
Secondary Antibody
- Name
- HRP-conjugated AffiniPure goat anti-mouse IgG, Fcγ subclass 2a specific
- Source
- Jackson ImmunoResearch Labs
- Catalog Number
- 115-035-206
- Concentration
- 1 µg/mL
- Time
- Overnight
- Temperature
- Room Temperature
Additional Information
- Protocol
- mAb3D Atlas thick-section based antibody screening protocol (Link opens in a new window)
- Controls
- The labeling pattern in individuals with Alzheimer's or Hoarding disorder (APP expression expected) were compared to an individual with diabetes and cancer (APP expression not expected).
Results
- Result
- Pass (The antibody worked under these conditions)
- Describe Results
- Observed labeling in individuals with Alzheimer's or Hoarding disorder but not in an individual with diabetes and cancer.