Anti-Ankyrin-B [N105/13R] in Western Blot (Rat)
Addgene #184191
- Data Submitted By
- James Trimmer
- Lab Name
- James Trimmer, UC Davis
- Submission Date
- February 06, 2024
- Publication Date
- February 06, 2024 (modified February 06, 2024)
- Abstract
- Experimental results for Anti-Ankyrin-B [N105/13R] (Addgene Antibody 184191) in a western blot assay with a rat sample.
Image attribution: James Trimmer, UC Davis
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-Ankyrin-B [N105/13R]
Addgene #184191 -
Mix of HRP-conjugated goat anti-mouse IgG1, goat anti-mouse IgG2a, goat anti-mouse IgG2b
Jackson Labs #
Materials & Methods
Sample
- Species
- Rat
- Cell / Tissue Type
- Brain
Buffer
- Name
- TBS
- pH
- 7.5
Blocking
- Agent
- Milk
- Concentration
- 4%
- Time
- 45 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-Ankyrin-B [N105/13R]
- Source
- Addgene
- Catalog Number
- 184191
- Species
- Mouse
- Gene / Target
- ANK2
- Concentration
- 5 µg/mL
- Time
- 45 min
- Temperature
- Room Temperature
Secondary Antibody
- Name
- Mix of HRP-conjugated goat anti-mouse IgG1, goat anti-mouse IgG2a, goat anti-mouse IgG2b
- Source
- Jackson Labs
- Concentration
- 1:8333
- Time
- 45 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
Total rat brain extract was reduced and separated by denaturing SDS-PAGE
- Protocol
- Trimmer lab immunoblot labeling protocol (Link opens in a new window)
- Predicted Molecular Weight
- >200 kDa for full-length protein, >160 kDa and smaller fragments possible due to proteolysis and variable sample preparation
Results
- Result
- Pass (The antibody worked under these conditions)