IHC-KO for Anti-IGDCC3 (PUNC) [IPI-mIGDC3.13]
Addgene #241897
- Data Submitted By
- Alexander Jaworski
- Lab Name
- Alexander Jaworski, Brown University
- Submission Date
- June 03, 2024
- Publication Date
- March 06, 2026 (modified March 06, 2026)
- DOI
- https://doi.org/10.57733/addgene.5co5wr
- Abstract
- Brachial-level transverse sections of E12.5 wild-type and Punc -/- (Punc KO) embryos were labeled with Anti-IGDCC3 (PUNC) [IPI-mIGDC3.13] (Addgene #241897). No labeling is observed on Punc -/- sections.
- Citation
-
IHC-KO for Anti-IGDCC3 (PUNC) [IPI-mIGDC3.13]. Jaworski A 2026. Addgene Report, https://doi.org/10.57733/addgene.5co5wr
![Brachial-level transverse sections of E12.5 wild-type and Punc -/- (Punc KO) embryos were labeled with Anti-IGDCC3 (PUNC) [IPI-mIGDC3.13]. In wild type, Punc is detected in the spinal cord mantle layer, including the ventral horn, as well as the DRG and motor and sensory axons. No labeling is observed on Punc -/- sections.](https://datahub-media.addgene.org/report-images/5co5wr/IPI-mIGDC3.13_IHC2.png)
Image attribution: Alexander Jaworski, Brown University
![Brachial-level transverse sections of E12.5 wild-type and Punc -/- (Punc KO) embryos were labeled with Anti-IGDCC3 (PUNC) [IPI-mIGDC3.13]. In wild type, Punc is detected in the spinal cord mantle layer, including the ventral horn, as well as the DRG and motor and sensory axons. No labeling is observed on Punc -/- sections.](https://datahub-media.addgene.org/report-images/5co5wr/IPI-mIGDC3.13_IHC1.png)
Image attribution: Alexander Jaworski, Brown University
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-IGDCC3 (PUNC) [IPI-mIGDC3.13]
Addgene #241897 -
Alexa 647-conjugated donkey anti-rabbit
Invitrogen #A-31573
Materials & Methods
Sample
- Target Species
- Mouse
- Cell / Tissue Type
- Spinal cord
- Additional Information
- 150 μm sections
Primary Antibody
- Name
- Anti-IGDCC3 (PUNC) [IPI-mIGDC3.13]
- Source
- Addgene
- Catalog Number
- 241897
- Host Species
- Rabbit
- Target Antigen
- IGDCC3
- Concentration
- 1:200
Secondary Antibody
- Name
- Alexa 647-conjugated donkey anti-rabbit
- Source
- Invitrogen
- Catalog Number
- A-31573
- Concentration
- 1:200 of 2 mg/mL stock
Additional Information
- Additional Information
All spinal cord sections were collected from brachial level. Immunohistochemistry (IHC) on 150 μm-thick vibratome sections, 2D neural tissue explants, collagen gel COS-7 cell cultures, and Dunn chamber cultures were described previously (Suter et al., 2020), with blocking buffer used for IHC on Dunn chamber cultures, and vibratome sections altered to include 2.5% donkey serum in lieu of bovine serum albumin.
Primary antibodies used for IHC were goat polyclonal antibodies against Islet1/2 (DHSB, 1:200) and Anti-IGDCC3 (PUNC) [IPI-mIGDC3.13] (Addgene, 1:200).
Secondary antibodies used were Alexa 488-conjugated donkey anti-goat (Invitrogen, A-11055) at a 1:200 dilution of a 2 mg/mL stock for the detection of Anti-goat Islet1/2, and Alexa 647-conjugated donkey anti-rabbit (Invitrogen, A-31573) at a 1:200 dilution of a 2 mg/mL stock for the detection of Anti-IGDCC3 (PUNC) [IPI-mIGDC3.13]. Images were acquired on a Nikon Ti-E microscope or Nikon CSU-W1 SoRa Ti2-E microscope.
References
Suter et al. (2020). TAG-1 multifunctionality coordinates neuronal migration, axon guidance, and fasciculation. Cell Rep, 30(4):1164-1177. https://doi.org/10.1016/j.celrep.2019.12.085 (Link opens in a new window) PMID: 31995756 (Link opens in a new window)
Results
- Result
- Pass (The antibody worked under these conditions)
- Describe Results
- Signal for IGDCC3 abolished in KO tissue
- Knockout Validation
- Yes