Anti-Arl13b [N295B/66R] - Chimeric in Western Blot (Rat)
Addgene #201860
- Data Submitted By
- Meghan Rego
- Lab Name
- Addgene
- Submission Date
- February 06, 2024
- Publication Date
- February 06, 2024 (modified February 06, 2024)
- Abstract
- Experimental results for Anti-Arl13b [N295B/66R] - Chimeric (Addgene Antibody 201860) in a western blot assay with a rat sample.
![Crude rat membranes immunoblotted against Anti-Arl13b [N295B/66R] recombinant mouse antibody (180085) or the recombinant chimeric rat antibody (201860).](https://datahub-media.addgene.org/report-images/510iwp/201860-Western_Blot-2023-07-17_132852.219372.png)
Image attribution: Addgene
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-Arl13b [N295B/66R] - Chimeric
Addgene #201860 -
Goat anti-mouse IgG (H+L) HRP
Thermo Fisher #A16078
Materials & Methods
Sample
- Species
- Rat
- Cell / Tissue Type
- Brain
- Total Amount
- 30 µg
Buffer
- Name
- TBS-T
- pH
- 7.4
Blocking
- Agent
- Milk
- Concentration
- 5%
- Time
- 45 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-Arl13b [N295B/66R] - Chimeric
- Source
- Addgene
- Catalog Number
- 201860
- Species
- Rat
- Gene / Target
- Arl13b
- Concentration
- 2 µg/mL
- Time
- 45 min
- Temperature
- Room Temperature
Secondary Antibody
- Name
- Goat anti-mouse IgG (H+L) HRP
- Source
- Thermo Fisher
- Catalog Number
- A16078
- Time
- 45 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
- Protocol
- Addgene Western Blot protocol
- Predicted Molecular Weight
- 60 kDa
Results
- Result
- Pass (The antibody worked under these conditions)