Anti-HCN2 [N71/37R] in Western Blot (Rat)

Hybridoma

Data Submitted By: James Trimmer
Lab Name: UC Davis/NIH NeuroMab Facility
Submission Date: February 06, 2024
Publication Date: February 06, 2024 (modified March 18, 2024)
Abstract: Experimental results for Anti-HCN2 [N71/37R] (hybridoma-derived) in a western blot assay with a rat sample.

Extracts of rat brain membrane (RBM) and of COS-1 cells transfected with GFPHCN1, GFP-HCN2 or Kv2.1 plasmids and probed with N71/37 (left), K89/34 (middle) or a control (right) TC supe. — Caption: Extracts of rat brain membrane (RBM) and of COS-1 cells transfected with GFPHCN1, GFP-HCN2 or Kv2.1 plasmids and probed with N71/37 (left), K89/34 (middle) or a control (right) TC supe.
Image attribution: UC Davis/NIH NeuroMab Facility

Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)]. — Caption: Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].
Image attribution: UC Davis/NIH NeuroMab Facility

Any further use of this image requires permission from the lab who submitted the data.

Related Antibody: Anti-HCN2 [N71/37R]
Addgene #184178

Materials & Methods

Sample

Species: Rat
Cell / Tissue Type: Brain

Primary Antibody

Name: Anti-HCN2 [N71/37R]
Species: Mouse
Gene / Target: Hcn2

Additional Information

Additional Information: NeuroMab hybridoma clone datasheet (Link opens in a new window)

Results

Result: Pass (The antibody worked under these conditions)