Anti-DNA-directed RNA polymerase subunit beta' [PR-mAb NT73] in Western Blot
Addgene #203884
- Data Submitted By
- Meghan Rego
- Lab Name
- Addgene
- Submission Date
- February 06, 2024
- Publication Date
- February 06, 2024 (modified February 06, 2024)
- Abstract
- Experimental results for Anti-DNA-directed RNA polymerase subunit beta' [PR-mAb NT73] (Addgene Antibody 203884) in a western blot assay with a other sample.
Image attribution: Addgene
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-DNA-directed RNA polymerase subunit beta' [PR-mAb NT73]
Addgene #203884 -
Goat anti-mouse IgG (H+L) HRP
Thermo Fisher #A16078
Materials & Methods
Sample
- Species
- Other
- Cell / Tissue Type
- E. coli
Buffer
- Name
- TBS-T
- pH
- 7.4
Blocking
- Agent
- Milk
- Concentration
- 5%
- Time
- 45 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-DNA-directed RNA polymerase subunit beta' [PR-mAb NT73]
- Source
- Addgene
- Catalog Number
- 203884
- Species
- Mouse
- Gene / Target
- rpoC
- Concentration
- 5 µg/mL
- Time
- 45 min
- Temperature
- Room Temperature
Secondary Antibody
- Name
- Goat anti-mouse IgG (H+L) HRP
- Source
- Thermo Fisher
- Catalog Number
- A16078
- Time
- 45 min
- Temperature
- Room Temperature
Additional Information
- Additional Information
Supernatant from E. coli cells expressing rpoC-6xHis was reduced and separated by denaturing SDS-PAGE
- Protocol
- Addgene Western Blot protocol
- Predicted Molecular Weight
- 153 kDa
Results
- Result
- Pass (The antibody worked under these conditions)