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Anti-GABA(A)R, Alpha4 [N398A/34R] in Immunocytochemistry

Addgene #184208

Data Submitted By
James Trimmer
Lab Name
James Trimmer, UC Davis
Submission Date
February 06, 2024
Publication Date
February 06, 2024 (modified February 06, 2024)
Abstract
Experimental results for Anti-GABA(A)R, Alpha4 [N398A/34R] (Addgene Antibody 184208) in an immunocytochemistry assay with a other sample.
Caption: COS cells were transfected with an antigen plasmid expressing RFP-tagged GABA-A-R-Alpha4 and stained with hybridoma-derived antibody from the Trimmer Lab at UC Davis (N398A/34 mAb) or recombinant antibody from Addgene (N398A/34 R-mAb).
Image attribution: James Trimmer, UC Davis

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibodies Used
Anti-GABA(A)R, Alpha4 [N398A/34R]
Addgene #184208
Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 488
Thermo Fisher #A-21131

Materials & Methods

Sample

Species
Other
Cell / Tissue Type
Primate COS Cells

Buffer

Name
TBS
pH
7.5

Fixative

Fixative
3% Paraformaldehdye, +Ca, +Mg in PBS pH 7.4

Permeabilization

Agent
0.1% Triton X-100

Blocking

Agent
Milk
Concentration
4%
Time
45 min
Temperature
Room Temperature

Primary Antibody

Name
Anti-GABA(A)R, Alpha4 [N398A/34R]
Source
Addgene
Catalog Number
184208
Species
Mouse
Gene / Target
Gabra4
Concentration
2 µg/mL
Time
45 min
Temperature
Room Temperature
View in Addgene Catalog

Secondary Antibody

Name
Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 488
Source
Thermo Fisher
Catalog Number
A-21131
Concentration
1:2500
Time
45 min
Temperature
Room Temperature

Additional Information

Additional Information

COS cells were transfected with an antigen plasmid expressing RFP-tagged GABA-A-R-Alpha4.

Protocol
Trimmer lab immunocytochemistry protocol (Link opens in a new window)

Results

Result
Pass (The antibody worked under these conditions)