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Anti-Neurexin-2-alpha [IPI-mNRXN2a.10] in Surface Plasmon Resonance (SPR)

Addgene #237806

Data Submitted By
Murali Anuganti, Aditi Kachare, Travis Riedel and Deborah Moshinsky
Lab Name
Institute for Protein Innovation (IPI)
Submission Date
February 02, 2026
Publication Date
February 09, 2026 (modified March 13, 2026)
DOI
https://doi.org/10.57733/addgene.215uvm
Abstract
Experimental results for Anti-Neurexin-2-alpha [IPI-mNRXN2a.10] (Addgene #237806) in SPR assay with a mouse sample.
Citation
Anti-Neurexin-2-alpha [IPI-mNRXN2a.10] in Surface Plasmon Resonance (SPR). Riedel T, Moshinsky D, Anuganti M, Kachare A 2026. Addgene Report, https://doi.org/10.57733/addgene.215uvm
Surface Plasmon Resonance (SPR) kinetics analysis of the interaction between Anti-Neurexin-2-alpha [IPI-mNRXN2a.10] (Addgene #237806) and mouse NRXN2a. SPR binding kinetics were measured on a Carterra LSA using HC30M chips (Carterra, #4279) at 25 °C. Goat anti-rabbit IgG Fc (Jackson ImmunoResearch, #111-005-046) was immobilized via amine coupling, and test antibodies were captured using a 96-channel print-head. Antigens (400 nM to five lower concentrations, 2-fold dilutions) were injected in antigen buffer (20 mM HEPES pH 7.4, 150 mM NaCl, 1 mM CaCl₂, 1 mM MgCl₂, 0.005% Tween 80) with 300 s association/ dissociation phases and acid regeneration. Data (reference/buffer subtracted, smoothed) were globally fit to a 1:1 Langmuir model to derive ka, kd, and KD using Carterra Kinetics software v1.9.2.44.63, and replotted in OriginPro 2023b. Results show a high-affinity and specific binding event between the antibody and antigen.
Caption: Surface Plasmon Resonance (SPR) kinetics analysis of the interaction between Anti-Neurexin-2-alpha [IPI-mNRXN2a.10] (Addgene #237806) and mouse NRXN2a. SPR binding kinetics were measured on a Carterra LSA using HC30M chips (Carterra, #4279) at 25 °C. Goat anti-rabbit IgG Fc (Jackson ImmunoResearch, #111-005-046) was immobilized via amine coupling, and test antibodies were captured using a 96-channel print-head. Antigens (400 nM to five lower concentrations, 2-fold dilutions) were injected in antigen buffer (20 mM HEPES pH 7.4, 150 mM NaCl, 1 mM CaCl₂, 1 mM MgCl₂, 0.005% Tween 80) with 300 s association/ dissociation phases and acid regeneration. Data (reference/buffer subtracted, smoothed) were globally fit to a 1:1 Langmuir model to derive ka, kd, and KD using Carterra Kinetics software v1.9.2.44.63, and replotted in OriginPro 2023b. Results show a high-affinity and specific binding event between the antibody and antigen.

This report is made available under the Creative Commons Attribution 4.0 International License.

Antibody Used
Anti-Neurexin-2-alpha [IPI-mNRXN2a.10]
Addgene #237806

Materials & Methods

Sample

Target Species
Mouse

Primary Antibody

Name
Anti-Neurexin-2-alpha [IPI-mNRXN2a.10]
Source
Addgene
Catalog Number
237806
Host Species
Rabbit
Target Antigen
NRXN2a
Concentration
0.25 µg/mL (1:4,000 dilution)
Temperature
Room Temperature
View in Addgene Catalog

Results

Result
Pass (The antibody worked under these conditions)
Describe Results
Results show a high-affinity and specific binding event between the antibody and antigen