Anti-APP [6E10-A5] in Immunohistochemistry (Mouse)
Addgene #226376
- Data Submitted By
- Zhuhao Wu and Wei Wang
- Lab Name
- Zhuhao Wu, Weill Cornell Medicine
- Submission Date
- November 27, 2024
- Publication Date
- December 03, 2024 (modified December 12, 2024)
- DOI
- https://doi.org/10.57733/addgene.1lmbyy
- Abstract
- Experimental results for Anti-APP [6E10-A5] (Addgene #226376) in an immunohistochemistry assay with mouse brain samples.
- Citation
-
Anti-APP [6E10-A5] in Immunohistochemistry (Mouse). Wu Z, Wang W 2024. Addgene Report, https://doi.org/10.57733/addgene.1lmbyy
![Brain sections were labeled with Anti-APP [6E10-A5] from Addgene (226376-rAb). As expected, the labeling patterns in APP^NL-G-F knock-in transgenic mice confirms APP expression which is absent in the WT B6 mouse strain. Mouse genetic samples were generously shared by Gan Laboratory at WCM.](https://datahub-media.addgene.org/report-images/1lmbyy/6e10a5_mouse.png)
Image attribution: Zhuhao Wu and Wei Wang, Weill Cornell Medicine
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-APP [6E10-A5]
Addgene #226376 -
HRP-conjugated AffiniPure goat anti-mouse IgG, Fcγ subclass 2a specific
Jackson ImmunoResearch Labs #115-035-206
Materials & Methods
Sample
- Species
- Mouse
- Cell / Tissue Type
- Brain
- Additional Information
- 100 µm thick section.
- Preparation
- Free-floating
Buffer
- Name
- PBS
Fixative
- Fixative
- Formaldehyde/Paraformaldehyde
Permeabilization / Delipidation
- Delipidation
- Yes
Blocking
- Agent
- PTxwH buffer
Primary Antibody
- Name
- Anti-APP [6E10-A5]
- Source
- Addgene
- Catalog Number
- 226376
- Species
- Mouse
- Gene / Target
- APP
- Concentration
- 1 µg/mL
- Time
- Overnight
- Temperature
- 4 ˚C
Secondary Antibody
- Name
- HRP-conjugated AffiniPure goat anti-mouse IgG, Fcγ subclass 2a specific
- Source
- Jackson ImmunoResearch Labs
- Catalog Number
- 115-035-206
- Concentration
- 1 µg/mL
- Time
- Overnight
- Temperature
- Room Temperature
Additional Information
- Additional Information
- APPNL-G-F knock-in transgenic mice express an APP construct with a humanized Aβ region and which includes the Swedish “NL”, the Iberian “F”, and the Arctic “G” mutations.
- Protocol
- mAb3D Atlas thick-section based antibody screening protocol (Link opens in a new window)
- Controls
- The labeling pattern in APPNL-G-F knock-in transgenic mice (APP expression expected) was compared to WT B6 mice (APP expression not expected).
Results
- Result
- Pass (The antibody worked under these conditions)
- Describe Results
- Observed labeling in APPNL-G-F knock-in transgenic mice but not in WT B6 mice.