Anti-Iduna/RNF146 [N201/35R] in Immunohistochemistry (Rat)
Addgene #180112
- Data Submitted By
- James Trimmer
- Lab Name
- James Trimmer, UC Davis
- Submission Date
- February 06, 2024
- Publication Date
- February 06, 2024 (modified February 06, 2024)
- Abstract
- Experimental results for Anti-Iduna/RNF146 [N201/35R] (Addgene Antibody 180112) in an immunohistochemistry assay with a rat sample.
![Free-floating rat brain sections were fixed and stained with purified Anti-Iduna/RNF146 [N201/35R] or hybridoma-derived tissue culture supernatant. The staining patterns from antibody produced at Addgene (AR0045) were compared to UC Davis-derived antibody (TC Supernatant).](https://datahub-media.addgene.org/report-images/1igtu6/180112-Immunohistochemistry-2022-03-09_143530.665861.png)
Image attribution: James Trimmer, UC Davis
This report is made available under the Creative Commons Attribution 4.0 International License.
- Antibodies Used
-
Anti-Iduna/RNF146 [N201/35R]
Addgene #180112 -
mix of biotinylated goat anti-mouse IgG1, goat anti-mouse IgG2a, goat anti-mouse IgG2b
Jackson Labs #115-065-205, 115-065-206, 115-065-207
Materials & Methods
Sample
- Species
- Rat
- Cell / Tissue Type
- Brain
Fixative
- Fixative
- Formaldehyde/Paraformaldehyde
Permeabilization / Delipidation
- Agent
- PBS containing 1:2000 avidin and 0.5% Triton X-100
- Time
- 45 min
- Temperature
- Room Temperature
Blocking
- Agent
- Serum
- Concentration
- 0.5%
- Time
- 45 min
- Temperature
- Room Temperature
Primary Antibody
- Name
- Anti-Iduna/RNF146 [N201/35R]
- Source
- Addgene
- Catalog Number
- 180112
- Species
- Mouse
- Gene / Target
- Rnf146
- Concentration
- 1 µg/mL
- Time
- overnight
- Temperature
- 4 °C
Secondary Antibody
- Name
- mix of biotinylated goat anti-mouse IgG1, goat anti-mouse IgG2a, goat anti-mouse IgG2b
- Source
- Jackson Labs
- Catalog Number
- 115-065-205, 115-065-206, 115-065-207
- Concentration
- 0.5 µg/mL
- Time
- 1 hour
- Temperature
- Room Temperature
Additional Information
- Additional Information
Tissue type: Free-floating brain sections
Antigen-retrieval: No
- Protocol
- Trimmer lab immunohistochemistry protocol (Link opens in a new window)
Results
- Result
- Pass (The antibody worked under these conditions)